Extract solution for cell-free protein synthesis, method for cell-free protein synthesis using same and method for preparation of the extract solution
AssigneeShimadzu Corporation
InventorsShoken Higashide, Toru Ezure, Satoko Shiraki, Masaaki Ito
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Abstract
The present invention provides a novel extract solution for cell-free protein synthesis, which contains at least an extract derived from a Bombyx mori L. tissue and an exogenous mRNA, which is easy to prepare and which is capable of realizing cell-free protein synthesis that can synthesize glycoprotein, a cell-free protein synthesis method using it, and a method for preparing an extract solution for cell-free protein synthesis, which includes adding an exogenous mRNA to an extract derived from a Bombyx mori L. tissue.
Claims17
An extract solution for cell-free protein synthesis, which comprises at least an extract derived from a Bombyx mori L. tissue and an exogenous mRNA.
An extract solution for cell-free protein synthesis, which comprises at least an extract derived from a Bombyx mori L. tissue, an exogenous mRNA and a protease inhibitor, wherein the Bombyx mori L. tissue is a posterior silk gland of Bombyx mori L. larva, and the protease inhibitor is phenylmethanesulfonyl fluoride.
A liquid composition for cell-free protein synthesis, which comprises at least an extract derived from a Bombyx mori L. tissue and a protease inhibitor.
A method for preparing an extract solution for cell-free protein synthesis, which comprises adding an exogenous mRNA to an extract derived from a Bombyx mori L. tissue obtained by extracting from the Bombyx mori L. tissue using a solution for extraction.
Dependencies are inferred from the claim text.