Non-or minimally invasive monitoring methods

A method for detecting the presence or amount of an analyte present beneath a target skin or mucosal surface of an individual, said method comprising:

• (a) disrupting said target surface to create one or more passages in that surface sufficient to allow said analyte to flow, exude or otherwise pass from beneath the target surface to the target surface;
• (b) applying an interface material over said target surface;
• (c) placing an occlusive covering over said interface material and said target surface, wherein said covering has a moveable or resealable portion that can be displaced to provide access to said target surface without removing the entire covering from the target surface;
• (d) moving the moveable or resealable portion from a first closed position to a second position that allows access to said target surface;
• (e) contacting the interface material with a sensing apparatus that detects the presence or amount of said analyte at the target surface;
• and
• (f) moving the moveable or resealable portion back to its first closed position thereby covering said target surface.

A method for detecting the presence or amount of an analyte present beneath a target skin or mucosal surface of an individual, said method comprising:

• (a) disrupting said target surface to create one or more passages in that surface sufficient to allow said analyte to flow, exude or otherwise pass from beneath the target surface to the target surface;
• (b) placing an occlusive covering over said target surface thereby covering said target surface, wherein said covering has a moveable or resealable portion that can be displaced to provide access to said target surface without removing the entire covering from the target surface;
• (c) moving the moveable or resealable portion from a first closed position to a second position that allows access to said target surface;
• (d) sampling said target surface and then detecting said analyte ex vivo;
• and
• (e) moving the moveable or resealable portion back to its first closed position thereby covering said target surface.

A method for detecting the presence or amount of an analyte present beneath a target skin or mucosal surface of an individual, said method comprising:

• (a) disrupting said target surface to create one or more passages in that surface sufficient to allow access to said analyte at the target surface;
• (b) applying an interface material over said target surface;
• (c) placing an occlusive covering over said interface material and said target surface, wherein said covering has a moveable or resealable portion that can be displaced to provide access to said target surface without removing the entire covering from the target surface;
• (d) moving the moveable or resealable portion from a first closed position to a second position that allows access to said target surface;
• (e) sampling said interface material and then detecting said analyte in the sample ex vivo;
• and
• (f) moving the moveable or resealable portion back to its first closed position thereby covering said target surface.

A method of monitoring for an analyte present beneath a target skin or mucosal surface of an individual, said method comprising:

• (a) accelerating particles into and/or across said target surface, wherein the acceleration of said particles into or across the target surface is effective to create micro-passages that allow access to the analyte at the target surface, and further wherein said particles are accelerated toward the target surface using a needleless syringe device or a particle-mediated delivery device;
• (b) attaching an occlusive adhesive patch having a resealable aperture to a surface surrounding the target surface, thereby covering said target surface with said patch, wherein said aperture circumscribes said target surface, and further wherein said aperture is closed;
• (c) opening said resealable aperture;
• (d) contacting the target surface with a sensor;
• (e) determining the presence or concentration of said analyte at the target surface;
• and
• (f) resealing said aperture, thereby maintaining hydration and allowing for continual monitoring over time.

A method of monitoring for an analyte present beneath a target skin or mucosal surface of an individual, said method comprising:

• (a) accelerating particles into and/or across said target surface, wherein the acceleration of said particles into or across the target surface is effective to allow passage of a fluid sample from beneath the target surface to the target surface, and further wherein said particles are accelerated toward the target surface using a needleless syringe device or a particle-mediated delivery device;
• (b) contacting said target surface with an interface medium, wherein the interface medium collects said fluid sample;
• (c) attaching an occlusive adhesive patch having a resealable aperture to a surface surrounding the target surface, thereby covering said target surface with said patch, wherein said aperture circumscribes said target surface, and further wherein said aperture is closed;
• (d) opening said resealable aperture;
• (e) contacting said interface medium with a sensor;
• (f) determining the presence or concentration of said analyte in the interface medium;
• and
• (g) resealing said aperture, thereby maintaining hydration and allowing for continual monitoring over time.