Interference reduction by rheumatoid factors
OwnerRainer Wehner, Frederic Donie +1inventors
InventorsRainer Wehner, Frederic Donie, Beatus Ofenloch-Hahnle
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Abstract
The invention concerns a method f or the determination of an analyte in which rheumatoid factors or rheumatoid-factor-like substances are added as an interference reducing reagent to reduce or avoid a hook effect The invention in addition concerns suitable reagent kits for carrying out the method.
Claims25
Method for the determination of an analyte in a sample,
- wherein
- rheumatoid factors or rheumatoid-factor-like substances are added to reduce interference.
Method as claimed in one of the previous claims,
- wherein
- it comprises the binding of the analyte to a solid phase.
Method as claimed in one of the previous claims,
- wherein
- it comprises the steps:
- (a) contacting the sample with a solid phase, a first analyte-specific receptor which is bound to the solid phase or is capable of binding to the solid phase, and a second analyte-specific receptor which carries a signal-generating group or is capable of binding to a signal-generating group and
- (b) detecting the presence or/and amount of the analyte by determining the signal-generating group on the solid phase.
Method as claimed in one of the previous claims,
- wherein
- it comprises an immunological reaction.
Method as claimed in one of the previous claims,
- wherein
- the analyte is selected from the group comprising antibodies and antigens.
Method as claimed in one of the previous claims,
- wherein
- the analyte is detected using a signal-generating group selected from radiolabels, enzymes, dyes, fluorescent groups and electrochemiluminescent groups.
Method as claimed in one of the previous claims,
- wherein
- the rheumatoid factors or rheumatoid-factor-like substances are present at a final concentration of 1 to 1000 TU/ml test mixture.
Method as claimed in one of the previous claims,
- wherein
- the rheumatoid factors or rheumatoid-factor-like substances are added in a soluble form.
Method as claimed in one of the previous claims,
- wherein
- the rheumatoid factors or rheumatoid-factor-like substances are used in a form bound to a solid phase or in a form which is capable of binding to a solid phase.
Method as claimed in one of the previous claims,
- wherein
- a specific receptor for the rheumatoid factors or rheumatoid-factor-like substances is additionally used which is bound to a solid phase or capable of binding to a solid phase.
Use of rheumatoid factors or rheumatoid-factor-like substances as interference reducing reagents in a detection method.
Method for reducing interference in immunoassays by adding rheumatoid factors or rheumatoid-factor-like substances.
Reagent kit for the determination of an analyte,
- wherein
- it contains rheumatoid factors or rheumatoid-factor-like substances as an interference reducing reagent.
Dependencies are inferred from the claim text.