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OwnerJeffrey K Horton, John a Smith +3inventors
InventorsJeffrey K Horton, John a Smith, Michelle L Teear, Jonathan M Kendall, Nigel P Michael
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Disclosed is a carrier peptide and a conjugate comprising a carrier peptide and a target molecule and a method for delivery of the target molecule into a cell. The carrier peptide contains from 10-15 amino acids, having a core sequence of 3-5 hydrophobic amino acids flanked by flanking amino acid sequences. The core sequence comprises residues selected from proline and leucine such that there is at least one each of proline and leucine and is symmetrical about an amino acid or a bond. Also disclosed is a method for delivery of a target molecule into a cell and a method for measuring a cellular process.
A carrier peptide for transport of a target molecule across a cell membrane and into a cell, the carrier peptide being selected from the group consisting of:
An isolated polynucleotide comprising a sequence encoding a peptide comprising the amino acid sequence according to any one of SEQ.ID No.3 to SEQ ID No.8.
A conjugate for delivery of a target molecule into a cell said conjugate comprising a carrier peptide having from 10-15 amino acids and having a core sequence of 3-5 hydrophobic amino acids flanked by flanking amino acid sequences, characterised in that said core sequence comprises residues selected from proline and leucine such that there is at least one of each of proline and leucine and that the core sequence is symmetrical about an amino acid or a bond, covalently bonded to an environmentally sensitive fluorescent dye.
A conjugate for delivery of a target molecule into a cell said conjugate comprising a carrier peptide having from 10-15 amino acids and having a core sequence of 3-5 hydrophobic amino acids flanked by flanking amino acid sequences, characterised in that said core sequence comprises residues selected from proline and leucine such that there is at least one of each of proline and leucine and that the core sequence is symmetrical about an amino acid or a bond, covalently bonded to a reporter wherein said reporter is a Green fluorescent protein (GFP) or analogues thereof.
A peptide according to claims 21 or 22 wherein said core sequence is selected from:
Dependencies are inferred from the claim text.