Novel assay
OwnerAlan Wise, Andrew James Browninventors
Life of this patent
Estimating the term…
Abstract
This invention relates to a method for identification of an agent that modulates activity of G-protein coupled receptor 41 (GPR 41), or G-protein coupled receptor 42 (GPR 42) which method comprises: (i) contacting a test agent with GPR 41, GPR42 or a variant of either thereof which is capable of coupling to a G-protein; and (ii) monitoring for GPR 41 or GPR 42 activity in the presence of a G-protein; thereby determining whether the test agent modulates GPR 41 or GPR 42 activity. An agent identifiable by this method is provided for use in the treatment of dyslipidaemia, coronary heart disease, atheroselerosis, thrombosis or obesity, angina, chronic renal failure, peripheral vascular disease, stroke, type II diabetes or metabolic syndrome (syndrome X).
Claims18
A method for identification of an agent that modulates activity of G-protein coupled receptor 41 (GPR 41), or G-protein coupled receptor 42 (GPR 42) which method comprises:
- (i) contacting a test agent with GPR 41, GPR 42 or a variant of either thereof which is capable of coupling to a G-protein;
- and
- (ii) monitoring for GPR 41 or GPR 42 activity in the presence of a G-protein;
- thereby determining whether the test agent modulates GPR 41 or GPR 42 activity.
A method according claim I wherein the test agent is contacted in step (i) with cells that express GPR 41, GPR 42 or a said variant of either thereof.
A method according to claim I wherein the test agent is contacted in step (i) with the membrane of cells that express GPR 41, GPR 42 or a said variant of either thereof.
A method according to any one of the preceding claims wherein the variant has at least 70% sequence identity to SEQ ID NO:
- 2 or SEQ ID NO:
- 4.
A method according to any one of the preceding claims wherein the G-protein is Gi-protein.
A test kit suitable for identification of an agent that modulates GPR 41 or GPR 42 activity, which kit comprises:
- (a) GPR 41, GPR 42 or a variant of either thereof which is capable of coupling to a Gi-protein;
- and
- (b) means for monitoring GPR 41 or GPR 42 activity.
Dependencies are inferred from the claim text.