Antigenic peptides, such as for G protein-coupled receptors (GPCRS), antibodies thereto, and systems for identifying such antigenic peptides
OwnerGlenna C. Burmer, Christine L. Roush +1inventors
InventorsGlenna C. Burmer, Christine L. Roush, Joseph P. Brown
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Abstract
The present invention provides antigenic peptides for GPCRs and antibodies relating thereto, and related systems, methods, compositions, and the like, such as diagnostics and medicaments. Where antibodies against a given GPCR are not known, the present invention provides such antibodies, and preferred antigenic sequences for producing such antibodies. Where antibodies against a given GPCR are known, the present invention provides preferred antigenic peptides for producing antibodies that exhibit improved specificity, affinity or capacity to perform antibody-related actions relative to the known antibodies.
Claims66
An isolated antigenic peptide according to any one of SEQ ID NOS. 692-2292.
An isolated antigenic peptide comprising an amino acid sequence that is at least about 90% identical to a sequence set forth in any one of SEQ ID NOS. 692-2292.
An isolated antigenic peptide that is an analog of an antigenic peptide according to any one of SEQ ID NOS. 692-2292.
An isolated antigenic peptide comprising a short antigenic amino acid sequence that is identical to at least 5 consecutive amino acids set forth in any one of SEQ ID NOS. 692-2292.
An isolated antigenic peptide comprising a short antigenic amino acid sequence that is identical to or contains no more than one conservative amino acid substitution over at least 7 consecutive amino acids set forth in any one of SEQ ID NOS. 692-2292.
An isolated antibody having high specificity and high affinity or avidity for a particular GPCR comprising a peptide sequence that is identical to any one of SEQ ID NOS. 692-703, 713-730, 744-802, 807-820, 825-875, 880-889, 917-941, 950-964, 971-984, 989-993, 1010-1013, 1021-1024, 1029-1043, 1049-1052, 1057-1072, 1087-1113, 1124-1151, 1161-1172, 1179-1187, 1198-1209, 1228-1231, 1245-1257, 1271-1279, 1304-1308, 1369-1372, wherein the antibody was produced using an isolated antigenic peptide comprising the peptide sequence that is identical to the any one of SEQ ID NOS. 692-703, 713-730, 744-802, 807-820, 825-875, 880-889, 917-941, 950-964, 971-984, 989-993, 1010-1013, 1021-1024, 1029-1043, 1049-1052, 1057-1072, 1087-1113, 1124-1151, 1161-1172, 1179-1187, 1198-1209, 1228-1231, 1245-1257, 1271-1279, 1304-1308, 1369-1372.
An isolated antibody having high specificity and high affinity or avidity for a particular GPCR comprising a peptide sequence that is at least about 90% identical to any one of SEQ ID NOS. 692-703, 713-730, 744-802, 807-820, 825-875, 880-889, 917-941, 950-964, 971-984, 989-993, 1010-1013, 1021-1024, 1029-1043, 1049-1052, 1057-1072, 1087-1113, 1124-1151, 1161-1172, 1179-1187, 1198-1209, 1228-1231, 1245-1257, 1271-1279, 1304-1308, 1369-1372, wherein the antibody was produced using the peptide sequence that is at least about 90% identical to the any one of SEQ ID NOS. 692-703, 713-730, 744-802, 807-820, 825-875, 880-889, 917-941, 950-964, 971-984, 989-993, 1010-1013, 1021-1024, 1029-1043, 1049-1052, 1057-1072, 1087-1113, 1124-1151, 1161-1172, 1179-1187, 1198-1209, 1228-1231, 1245-1257, 1271-1279, 1304-1308, 1369-1372.
An isolated antibody having high specificity and high affinity or avidity for a particular GPCR comprising a peptide sequence that is an analog to any one of SEQ ID NOS. 692-703, 713-730, 744-802, 807-820, 825-875, 880-889, 917-941, 950-964, 971-984, 989-993, 1010-1013, 1021-1024, 1029-1043, 1049-1052, 1057-1072, 1087-1113, 1124-1151, 1161-1172, 1179-1187, 1198-1209, 1228-1231, 1245-1257, 1271-1279, 1304-1308, 1369-1372, wherein the antibody was produced using an isolated antigenic peptide comprising the peptide sequence that is the analog to the any one of SEQ ID NOS. 692-703, 713-730, 744-802, 807-820, 825-875, 880-889, 917-941, 950-964, 971-984, 989-993, 1010-1013, 1021-1024, 1029-1043, 1049-1052, 1057-1072, 1087-1113, 1124-1151, 1161-1172, 1179-1187, 1198-1209, 1228-1231, 1245-1257, 1271-1279, 1304-1308, 1369-1372.
An isolated antibody having high specificity and high affinity or avidity for a particular GPCR comprising a peptide sequence that is identical to at least 5 consecutive amino acids set forth any one of SEQ ID NOS. 692-703, 713-730, 744-802, 807-820, 825-875, 880-889, 917-941, 950-964, 971-984, 989-993, 1010-1013, 1021-1024, 1029-1043, 1049-1052, 1057-1072, 1087-1113, 1124-1151, 1161-1172, 1179-1187, 1198-1209, 1228-1231, 1245-1257, 1271-1279, 1304-1308, 1369-1372, wherein the antibody was produced using a short isolated antigenic peptide comprising the at least 5 consecutive amino acids set forth in the any one of SEQ ID NOS. 692-703, 713-730, 744-802, 807-820, 825-875, 880-889, 917-941, 950-964, 971-984, 989-993, 1010-1013, 1021-1024, 1029-1043, 1049-1052, 1057-1072, 1087-1113, 1124-1151, 1161-1172, 1179-1187, 1198-1209, 1228-1231, 1245-1257, 1271-1279, 1304-1308, 1369-1372.
An isolated antibody specific for a particular GPCR comprising a peptide sequence that is identical to any one of SEQ ID NOS. 704-712, 731-743, 774-777, 803-806, 821-824, 876-879, 890-916, 942-949, 965-970, 985-988, 994-1009, 1014-1020, 1025-1028, 1044-1048, 1053-1056, 1073-1086, 1114-1123, 1152-1160, 1173-1178, 1188-1197, 1210-1227, 1232-1244, 1258-1270, 1280-1303, 1309-1368, 1373-1377, 1386-1389, 1394-1402, 1462-1482, 1496-1525, 1542-1549, 1557-1563, 1583-1649, 1656-1679, 1684-1688, 1693-1732, 1744-1752, 1765-1839, 1846-1854, 1855-1866, 1871-1917, 1926-1941, 1952-1955, 1960-1980, 1985-2141, 2152-2165, and 2170-2292, wherein the antibody was produced using an isolated antigenic peptide comprising the peptide sequence that is identical to the any one of SEQ ID NOS. 704-712, 731-743, 774-777, 803-806, 821-824, 876-879, 890-916, 942-949, 965-970, 985-988, 994-1009, 1014-1020, 1025-1028, 1044-1048, 1053-1056, 1073-1086, 1114-1123, 1152-1160, 1173-1178, 1188-1197, 1210-1227, 1232-1244, 1258-1270, 1280-1303, 1309-1368, 1373-1377, 1386-1389, 1394-1402, 1462-1482, 1496-1525, 1542-1549, 1557-1563, 1583-1649, 1656-1679, 1684-1688, 1693-1732, 1744-1752, 1765-1839, 1846-1854, 1855-1866, 1871-1917, 1926-1941, 1952-1955, 1960-1980, 1985-2141, 2152-2165, and 2170-2292.
An isolated antibody specific for a particular GPCR comprising a peptide sequence that is at least about 90% identical to any one of SEQ ID NOS. 704-712, 731-743, 774-777, 803-806, 821-824, 876-879, 890-916, 942-949, 965-970, 985-988, 994-1009, 1014-1020, 1025-1028, 1044-1048, 1053-1056, 1073-1086, 1114-1123, 1152-1160, 1173-1178, 1188-1197, 1210-1227, 1232-1244, 1258-1270, 1280-1303, 1309-1368, 1373-1377, 1386-1389, 1394-1402, 1462-1482, 1496-1525, 1542-1549, 1557-1563, 1583-1649, 1656-1679, 1684-1688, 1693-1732, 1744-1752, 1765-1839, 1846-1854, 1855-1866, 1871-1917, 1926-1941, 1952-1955, 1960-1980, 1985-2141, 2152-2165, and 2170-2292, wherein the antibody was produced using the peptide sequence that is at least about 90% identical to the any one of SEQ ID NOS. 704-712, 731-743, 774-777, 803-806, 821-824, 876-879, 890-916, 942-949, 965-970, 985-988, 994-1009, 1014-1020, 1025-1028, 1044-1048, 1053-1056, 1073-1086, 1114-1123, 1152-1160, 1173-1178, 1188-1197, 1210-1227, 1232-1244, 1258-1270, 1280-1303, 1309-1368, 1373-1377, 1386-1389, 1394-1402, 1462-1482, 1496-1525, 1542-1549, 1557-1563, 1583-1649, 1656-1679, 1684-1688, 1693-1732, 1744-1752, 1765-1839, 1846-1854, 1855-1866, 1871-1917, 1926-1941, 1952-1955, 1960-1980, 1985-2141, 2152-2165, and 2170-2292.
An isolated antibody specific for a particular GPCR comprising a peptide sequence that is an analog to any one of SEQ ID NOS. 704-712, 731-743, 774-777, 803-806, 821-824, 876-879, 890-916, 942-949, 965-970, 985-988, 994-1009, 1014-1020, 1025-1028, 1044-1048, 1053-1056, 1073-1086, 1114-1123, 1152-1160, 1173-1178, 1188-1197, 1210-1227, 1232-1244, 1258-1270, 1280-1303, 1309-1368, 1373-1377, 1386-1389, 1394-1402, 1462-1482, 1496-1525, 1542-1549, 1557-1563, 1583-1649, 1656-1679, 1684-1688, 1693-1732, 1744-1752, 1765-1839, 1846-1854, 1855-1866, 1871-1917, 1926-1941, 1952-1955, 1960-1980, 1985-2141, 2152-2165, and 2170-2292, wherein the antibody was produced using an isolated antigenic peptide comprising the peptide sequence that is the analog to the any one of SEQ ID NOS. 704-712, 731-743, 774-777, 803-806, 821-824, 876-879, 890-916, 942-949, 965-970, 985-988, 994-1009, 1014-1020, 1025-1028, 1044-1048, 1053-1056, 1073-1086, 1114-1123, 1152-1160, 1173-1178, 1188-1197, 1210-1227, 1232-1244, 1258-1270, 1280-1303, 1309-1368, 1373-1377, 1386-1389, 1394-1402, 1462-1482, 1496-1525, 1542-1549, 1557-1563, 1583-1649, 1656-1679, 1684-1688, 1693-1732, 1744-1752, 1765-1839, 1846-1854, 1855-1866, 1871-1917, 1926-1941, 1952-1955, 1960-1980, 1985-2141, 2152-2165, and 2170-2292.
An isolated antibody specific for a particular GPCR comprising a peptide sequence that is identical to at least 5 consecutive amino acids set forth any one of SEQ ID NOS. 704-712, 731-743, 774-777, 803-806, 821-824, 876-879, 890-916, 942-949, 965-970, 985-988, 994-1009, 1014-1020, 1025-1028, 1044-1048, 1053-1056, 1073-1086, 1114-1123, 1152-1160, 1173-1178, 1188-1197, 1210-1227, 1232-1244, 1258-1270, 1280-1303, 1309-1368, 1373-1377, 1386-1389, 1394-1402, 1462-1482, 1496-1525, 1542-1549, 1557-1563, 1583-1649, 1656-1679, 1684-1688, 1693-1732, 1744-1752, 1765-1839, 1846-1854, 1855-1866, 1871-1917, 1926-1941, 1952-1955, 1960-1980, 1985-2141, 2152-2165, and 2170-2292, wherein the antibody was produced using a short isolated antigenic peptide comprising the at least 5 consecutive amino acids set forth in the any one of SEQ ID NOS. 704-712, 731-743, 774-777, 803-806, 821-824, 876-879, 890-916, 942-949, 965-970, 985-988, 994-1009, 1014-1020, 1025-1028, 1044-1048, 1053-1056, 1073-1086, 1114-1123, 1152-1160, 1173-1178, 1188-1197, 1210-1227, 1232-1244, 1258-1270, 1280-1303, 1309-1368, 1373-1377, 1386-1389, 1394-1402, 1462-1482, 1496-1525, 1542-1549, 1557-1563, 1583-1649, 1656-1679, 1684-1688, 1693-1732, 1744-1752, 1765-1839, 1846-1854, 1855-1866, 1871-1917, 1926-1941, 1952-1955, 1960-1980, 1985-2141, 2152-2165, and 2170-2292.
An isolated nucleic acid molecule encoding an antigenic peptide according to any one of SEQ ID NOS. 692-2292.
An isolated nucleic acid molecule encoding an antigenic peptide that is at least about 90% identical to any one of the antigenic peptides set forth in SEQ ID NOS. 692-2292.
A process for producing an isolated polynucleotide comprising hybridizing a nucleotide encoding an antigenic peptide according to any one of SEQ ID NOS. 692-2292 to genomic DNA under highly stringent conditions and isolating the polynucleotide detected with the nucleotide.
A method of identifying an amino acid sequence for an antigenic peptide from a candidate polypeptide sequence wherein the antigenic peptide has a length of about 5 to about 100 amino acids, the method comprising:
- a) searching the candidate polypeptide sequence using a comparison window of the length, and
- b) selecting against amino acid sequences of the length and having at least 3 characteristics selected from the group consisting of 1) at least two consecutive prolines, 2) at least two consecutive serines, 3) at least two consecutive lysines, 4) at least two consecutive arginines, 5) at least two consecutive aspartic acids, 6) at least two consecutive glutamic acids, 7) methionine, 8) tryptophan, and 9) at least five consecutive amino acids comprising no charged amino acids.
Dependencies are inferred from the claim text.