Methods for the identification of inhibitors of thioredoxin expression or activity in plants

A method for identifying a compound as a candidate for a herbicide, comprising:

• a) contacting a compound with at least one polypeptide selected from the group consisting of:
• iii) the polypeptide set forth in SEQ ID NO:
• 2 or 4;
• and
• iv) a polypeptide have at least 80% sequence identity with the polypeptide set forth in SEQ ID NO:
• 2 or 4;
• and
• b) detecting the presence and/or absence of binding between said compound and said polypeptide;
• wherein binding indicates that said compound is a candidate for a herbicide.

A method for identifying a compound as a candidate for a herbicide, comprising:

• a) contacting an oxidized target protein with TRX;
• b) contacting said oxidized target protein with TRX and said candidate compound;
• and
• c) determining the concentration of at least one of oxidized target protein, and/or reduced target protein after the contacting of steps (a) and (b), wherein a higher concentration of a substrate (oxidized target protein) and/or a lower level of a product (reduced target protein) detected in the presence of the candidate compound (step b) than that detected in the absence of the compound (step a) indicates that said compound is a candidate for a herbicide.

A method for identifying a compound as a candidate for a herbicide, comprising:

• a) contacting oxidized target protein with a polypeptide selected from the group consisting of:
• i) the polypeptide set forth in SEQ ID NO:
• 2 or 4;
• and
• ii) a polypeptide have at least 80% sequence identity with the polypeptide set forth in SEQ ID NO:
• 2 or 4;
• b) contacting said oxidized target protein with said polypeptide and said compound;
• and
• c) determining the concentration of at least one of oxidized target protein, and/or reduced target protein after the contacting of steps (a) and (b) wherein a higher concentration of a substrate (oxidized target protein) and/or a lower level of a product (reduced target protein) detected in the presence of the candidate compound (step b) than that detected in the absence of the compound (step a) indicates that said compound is a candidate for a herbicide.