Antibodies to crosslinkers and methods for using the same
OwnerMangalasseril G. Sarngadharan, Ranajit Pal +1inventors
InventorsMangalasseril G. Sarngadharan, Ranajit Pal, Anthony L. DeVico
Life of this patent
Estimating the term…
Abstract
The present invention is directed to the discovery of antibodies that will react to proteins or nucleic acids bound to particular crosslinkers, but not to the free crosslinkers or free proteins or nucleic acids. Monoclonal antibodies with such binding specificity have widespread applications in receptor-ligand binding, immunodiagnostic and nucleic acid diagnostics.
Claims14
A monoclonal antibody that will specifically bind to a BS3 or DSS modified protein or nucleic acid, but not to free BS3 or DSS or unmodified protein or nucleic acid.
A nucleic acid molecule which has been modified by covalent bonding to BS3 or DSS.
A method of assaying receptor-ligand binding, comprising:
- (a) linking BS3 or DSS to the ligand of interest;
- (b) reacting the BS3 or DSS modified ligand with cells expressing the ligand's receptor;
- (c) reacting bound BS3 or DSS modified ligand/receptor with labeled anti-BS3/DSS monoclonal antibody;
- and
- (d) detecting the label on a complex formed between the labeled antibody and the receptor/ligand.
A method of assaying receptor-ligand binding, comprising:
- (a) linking BS3 or DSS to the ligand of interest;
- (b) reacting the BS3 or DSS modified ligand with cells expressing the ligand's receptor;
- (c) reacting bound BS3 or DSS modified ligand/receptor with anti-BS3/DSS monoclonal antibody, followed by anti-mouse IgG conjugated to FITC;
- and
- (d) detecting the bound FITC-conjugated antibody by flow cytometry or immunofluorescence.
A method for detecting products of a nucleic acid amplification, comprising:
- (a) labelling NTPs that contain a free NH2 group with BS3 or DSS;
- (b) using the labelled NTPs in an amplification reaction mixture;
- (c) at the conclusion of the amplification, reacting the amplification products with anti-BS3/DSS antibody, which is itself labelled with a detectable moiety or reacted with a labelled antibody that will bind to the anti-BS3/DSS antibody, thereby detecting the amplification products.
A method for detecting a nucleic acid sequence, comprising hybridizing a probe with complementary regions of said sequence, wherein said probe comprises a nucleic acid sequence labeled with BS3 or DSS.
An immunoassay using a labeled antigen or a labeled antibody, wherein BS3 or DSS is the label.
A method for detecting an antigen of interest in a mixture of antigens, comprising:
- (a) modifying the antigens in the sample with BS3 or an analogue thereof;
- (b) reacting the modified antigens with a capture antibody, which is an IgA anti-BS3 antibody;
- (c) reacting the bound antigen with an IgG antibody that is specific to the antigen of interest;
- and
- (d) detecting bound IgG by reacting with a labeled anti-IgG antibody,
- thereby detecting the antigen of interest.
Dependencies are inferred from the claim text.