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US 2003/0113779 A1Published application
Screen for compounds with affinity for nucleic acids
OwnerJaime E. Arenas, James W. Lillie +1inventors
InventorsJaime E. Arenas, James W. Lillie, Andrew Pakula
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Abstract
The present invention provides methods for high-throughput screening for bioactive compounds, in particular those that bind to RNA sequences involved in the pathogenesis of disease or in regulation of a physiological function. The methods involve measuring the conformation of an RNA target in the presence and absence of test ligands, and identifying as a ligand any test ligand that causes a measurable change in target RNA conformation.
Claims9
A high-throughput method for identifying a ligand that binds a predetermined target RNA sequence, which comprises:
- (a) selecting as test ligands a plurality of compounds not known to bind to the target RNA sequence;
- (b) incubating the target RNA sequence in the presence of each of said test ligands to produce a test combination;
- (c) incubating the target RNA sequence in the absence of a test ligand to produce a control combination;
- (d) measuring the conformation of the target RNA sequence in each combination;
- (e) selecting as a ligand any test ligand that causes a measurable change in the target RNA conformation in the test combination relative to the target RNA conformation in the control combination;
- and
- (f) repeating steps (b)-(c) with a plurality of said test ligands to identify a ligand that binds to the target RNA sequence.
4 more elements
Dependencies are inferred from the claim text.