Rapid and specific detection of campylobacter
OwnerJohn W. Czajkainventor
Life of this patent
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Abstract
The present invention provides a method for specifically detecting pathogenic Campylobacter species in a complex sample. The target pathogenic Campylobacter species can be Campylobacter jejuni or Campylobacter coli. The complex sample can be a food sample, water sample, or selectively enriched food matrix. The method of detection utilizes PCR amplification with, or without, an internal positive control, and appropriate primer pairs. Multiple species can be detected in the same reaction. The reagents necessary to perform the method can be supplied as a kit and/or in tablet form.
Claims13
A method for detecting a pathogenic Campylobacter species in a sample, the method comprising:
- (a) preparing the sample for PCR amplification
- (b) performing PCR amplification of the sample using a combination of PS1 (SEQ ID NOs:
- 1 and 2) and PS2 (SEQ ID NOs:
- 3 and 4) primers;
- and
- (c) examining the PCR amplification result,
- whereby a positive amplification indicates the presence of a pathogenic Campylobacter species.
A method for detecting Campylobacter coli in a sample, the method comprising:
- (a) preparing the sample for PCR amplification
- (b) performing PCR amplification of the sample using PS1 primers (SEQ ID NOs:
- 1 and 2);
- and
- (c) examining the PCR amplification result,
- whereby a positive amplification indicates the presence of a pathogenic Campylobacter coli in the sample.
A method for detecting Campylobacter jejuni in a sample, the method comprising:
- (a) preparing the sample for PCR amplification
- (b) performing PCR amplification of the sample using PS2 (SEQ ID NOs:
- 3 and 4) primers;
- and
- (c) examining the PCR amplification result,
- whereby a positive amplification indicates the presence of Campylobacter jejuni in the sample.
An isolated polynucleotide for the specific detection of Campylobacter coli, consisting essentially of the nucleic acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2.
An isolated polynucleotide for the specific detection of Campylobacter jejuni, consisting essentially of the nucleic acid sequence of SEQ ID NO:
- 3 or SEQ ID NO:
- 4.
A kit for the detection of a pathogenic Campylobacter species selected from the group consisting of Campylobacter jejuni and Campylobacter coli in a sample, the kit comprising:
- (a) at least one pair of PCR primers selected from the group consisting of PS1 (SEQ ID NOs:
- 1 and 2) and PS2 (SEQ ID NOs:
- 3 and 4);
- and
- (b) a mixture of suitable PCR reagents comprising a thermostable DNA polymerase.
Dependencies are inferred from the claim text.