Method for random cDNA amplification

A method for amplification of a pool of RNA sequences, comprising:

• a) synthesizing total first strand cDNA with a first primer, said first primer being an extendible oligonucleotide comprising a first segment located at the 3′ terminal part of said oligonucleotide which is capable of hybridizing to substantially all RNAs contained in the RNA pool and a second segment, said second segment beeing located more proximal to the 5′ end of said oligonucleotide as compared to said first segment, said second segment being capable of serving as a primer binding side for another nucleic acid amplification primer by itself;
• b) synthesizing total second strand cDNA with a second primer, said second primer being an extendible oligonucleotide comprising a first randomized segment located at the 3′ part of said primer and a second segment which is located more proximal to the 5′ end of said oligonucleotide as compared to said first segment, said second segment being capable of serving as a primer binding site by itself for a nucleic acid amplification primer such that a double stranded cDNA is generated;
• and
• c) amplification of said double stranded cDNA.