Oligonucleotide-modified ROMP polymers and co-polymers
OwnerChad A. Mirkin, SonBinh T. Nguyen +2inventors
InventorsChad A. Mirkin, SonBinh T. Nguyen, Keith J. Watson, So-Jung Park
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Abstract
Ring-opening metathesis polymerization (ROMP) polymers or copolymers having oligonucleotides bound thereto, materials comprised of the oligonucleotide-modified ROMP polymers, and methods of making and using the same for preparing new materials and for detection of target nucleic acids are disclosed.
Claims70
A ROMP polymer or co-polymer having oligonucleotides bound thereto.
A ROMP polymer comprising an oligonucleotide-modified product produced by the ROMP polymerization of monomer 2 to produce a homopolymer template and post-polymerization modification of the polymer template to attach oligonucleotides.
A ROMP co-polymer having oligonucleotides bound thereto produced by the process of (a) sequential block ROMP polymerization of monomer 2 and at least one or more different monomers to produce a ROMP co-polymer template;
- (b) post-polymerization modification of the template, followed by coupling of oligonucleotides to the modified template.
Materials or structures comprising a ROMP polymer or co-polymer having oligonucleotides bound thereto.
Materials or structures comprising a first and second ROMP polymers or copolymers having oligonucleotides bound thereto, the oligonucleotides bound to the first ROMP polymer or co-polymer having a sequence that is complementary to the oligonucleotides bound to the second ROMP polymer or co-polymer.
Materials or structures comprised of:
- (a) particles having oligonucleotides attached thereto;
- and
- (b) a connector for holding the particles together, the connector comprising a ROMP polymer or co-polymer having oligonucleotides bound thereto, the oligonucleotides bound to the ROMP polymer or co-polymer having a sequence complementary to at least a portion of the sequence of the oligonucleotides bound to the particles.
Materials or structures comprised of:
- (a) particles having oligonucleotides attached thereto, the oligonucleotides comprising at least one type of recognition oligonucleotides, each type of recognition oligonucleotides comprising a spacer portion and a recognition portion, the spacer portion having a functional group through which the spacer portion is bound to the particles, the recognition portion having a sequence complementary to at least one portion of the sequence of another oligonucleotide;
- and
- (b) a connector for holding the particles together, the connector comprising a ROMP polymer or co-polymer having oligonucleotides bound thereto, the oligonucleotides comprise a spacer portion and a recognition portion, wherein the spacer portion is bound to the ROMP polymer or co-polymer and the recognition portion has a sequence complementary to at least one portion of the sequence of the oligonucleotides bound to the particles.
Materials or structures comprised of:
- (a) particles having oligonucleotides attached thereto, the oligonucleotides comprising:
- (i) at least one type of recognition oligonucleotides, each type of recognition oligonucleotides comprising a spacer portion and a recognition portion, the spacer portion having a functional group through which the spacer portion is bound to the particles, the recognition portion having a sequence complementary to at least one portion of the sequence of another oligonucleotide;
- and
- (ii) a type of diluent oligonucleotides;
- and
- (b) a connector for holding the particles together, the connector comprising a ROMP polymer or co-polymer having oligonucleotides bound thereto, the oligonucleotides comprise a spacer portion and a recognition portion, wherein the spacer portion is bound to the ROMP polymer or co-polymer and the recognition portion having a sequence complementary to at least one portion of the sequence of the oligonucleotides bound to the particles.
Materials or structures comprised of:
- (a) at least two types of particles having oligonucleotides attached thereto, the first type of particle having at least two types of oligonucleotides, the first type of oligonucleotides bound to the first type of particles having a sequence that is complementary to at least a portion of the sequence of the oligonucleotides bound to a second type of particle;
- and
- (b) oligonucleotide polymer conjugates for holding the particles together, the oligonucleotide polymer conjugate comprising a ROMP polymer having oligonucleotides bound thereto, the oligonucleotides of the oligonucleotide polymer conjugate having a sequence complementary to at least one portion of the sequence of a second type of oligonucleotides bound to the first type of particles.
A method of fabrication comprising
- providing a ROMP polymer or co-polymer having at least one type of oligonucleotides bound thereto, the oligonucleotides having a selected sequence, the sequence of each type of oligonucleotide having at least two portions;
- providing one or more types of particle-oligonucleotide conjugates, the oligonucleotides attached to the particles of each of the types of conjugates having a sequence complementary to the sequence of a portion of a oligonucleotide bound to the ROMP polymer or co-polymer;
- and
- contacting the ROMP polymer or co-polymer and particle oligonupcleotide conjugates under conditions effective to allow hybridization of the oligonucleotides attached to the particles to the oligonucleotides bound to the ROMP polymer or co-polymer so that a desired material or structure is formed wherein the particles conjugates are held together by oligonucleotides bound to the ROMP polymer.
A method of fabrication comprising:
- providing at least two types of particle-oligonucleotide conjugates, the first type of particle-oligonucleotide conjugates have at least two types of oligonucleotides wherein the first type of oligonucleotides attached to the first type of particle-oligonucleotide conjugates has a sequence that is complementary to that of the oligonucleotides attached to the particles of the second type of conjugates, the second type of oligonucleotides attached to the particles of the first type of conjugates having a sequence that is complementary to that of the oligonucleotides attached to the particles of a second type of conjugates;
- providing a ROMP polymer or co-polymer having oligonucleotides bound thereto, the oligonucleotides having a sequence that is complementary to a second type of oligonucleotides bound to the first type of particle-oligonucleotide conjugates;
- contacting the first and second types of particle-oligonucleotide conjugates with the ROMP polymer or co-polymer under conditions effective to allow hybridization of the oligonucleotides on the first type of particle-oligonucleotide conjugates with the oligonucleotides on the second type of particle-oligonucleotide conjugates and on the ROMP polymer or co-polymer so that a desired material or structure is formed.
A method for preparing a ROMP polymer or co-polymer having oligonucleotides bound thereto, the method comprising:
- providing (i) a ROMP polymer or co-polymer modified with chlorophosphoramidite and (ii) oligonucleotides bound to a solid support;
- contacting the chlorophosphoramidite-modified ROMP polymer with the oligonucleotides bound to a support to produce an oligonucleotide ROMP polymer conjugate bound to the support;
- and
- cleaving the oligonucleotide-modified ROMP polymer or copolymer from the support.
A method of fabrication comprising:
- providing first and second ROMP polymers or co-polymers having oligonucleotides bound thereto, the oligonucleotides bound to the first ROMP polymer or co-polymer having a sequence that is complementary to the oligonucleotides bound to the second ROMP polymer or co-polymer;
- and
- contacting the first and second ROMP polymers or co-polymer under conditions effective to allow hybridization of the oligonucleotides on the first ROMP polymer or co-polymer with the oligonucleotides on the second ROMP polymer or co-polymer so that a desired material or structure is formed.
In a method for the detection of one or more target nucleic acids in a sample, the sequence of each nucleic acid having at least two portions, the method comprising:
- providing one or more types of oligonucleotide-modified ROMP polymer or copolymer, the sequence of the oligonucleotides bound to each type of polymer or copolymer has at least two portions wherein at least one portion of the sequence of the oligonucleotides is complementary to first portion of a sequence of a target nucleic acid, wherein the oligonucleotides bound to one type of polymer or copolymer is different from another type, wherein each type of polymer or copolymer serves as a unique identifier for a particular target nucleic acid, and wherein the polymer or copolymer includes electrochemical labels;
- providing a gold electrode surface having oligonucleotides bound thereto, the oligonucleotides that are bound to the surface have a sequence having at least two portions wherein the first portion of the oligonucleotides is complementary to a second portion of the target nucleic acid;
- contacting the one or more types of oligonucleotide-modified ROMP polymer or copolymer, the gold surface, and the sample under conditions effective to allow for hybridization of the oligonucleotides bound to the polymer or copolymer with the target nucleic acids and for hybridization of the oligonucleotides bound to the surface with the target nucleic acids to form a complex on the surface in the presence of one or more target nucleic acids;
- and
- electrochemically detecting for the presence of the complex,.
A kit for detecting a target nucleic acid in a sample, the kit comprising at least one or more containers including one or more types of chlorophosphoramidite modified ROMP polymer or copolymer, wherein each polymer or copolymer has a different redox activity and can be used for coupling with oligonucleotides.
A kit for detecting a target nucleic acid in a sample, the kit comprising at least one or more containers including one or more types of chlorophosphoramidite-modifiable ROMP polymer or copolymer, wherein each polymer or copolymer has a different redox activity and can serve as an identifier for a specific target nucleic acid.
A kit for detecting a target nucleic acid in a sample, the kit comprising at least one or more containers including one or more types of oligonucleotide-modified ROMP polymer or copolymer, wherein each polymer or copolymer has a different redox activity and serves as an identifier for a specific target nucleic acid.
A system for detecting one or more target nucleic acids in a sample, the sequence of target nucleic acids have at least two portions, in a sample comprising
- (a) one or more types of oligonucleotide-modified ROMP polymer or copolymer, wherein each polymer or copolymer has a different redox activity and serves as an identifier for a specific target nucleic acid, the oligonucleotides bound to one type of polymer or copolymer is different from another, the oligonucleotides have a sequence having at least two portions, one portion of the sequence of the oligonucleotides is complementary to a first portion of a target nucleic acid;
- (b) a gold electrode surface having oligonucleotides bound thereto wherein the oligonucleotides bound to the surface has a sequence that is complementary to a second portion of a target nucleic acid;
- and
- (c) a detector for electrochemical detection of one or more polymers or copolymers bound to the surface in the presence of one or more target nucleic acids.
Dependencies are inferred from the claim text.