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US 2003/0113738 A1Published application
Evolving new molecular function
AssigneePresident and Fellows of Harvard College
InventorsDavid R. Liu, Zev Gartner, Matthew W. Kanan
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AssigneePresident and Fellows of Harvard College
InventorsDavid R. Liu, Zev Gartner, Matthew W. Kanan
Estimating the term…
Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.
A method of syntheiszing one or more chemical compounds, the method comprising the steps of:
A library comprising one or more chemical compounds wherein each of the chemical compounds is bonded to an amplifiable template whose nucleotide sequence is informative of the structure of the chemical compounds.
A method for the synthesis of a library of chemical compounds, the method comprising the steps of:
A method for the synthesis of one or more unnatural polymers, the method comprising steps of:
The method of claim 23, wherein the desired property comprises catalyzing a chemical reaction.
A method of evolving a library of compounds, the method comprising steps of:
A kit comprising one or more nucleic acid templates and one or more transfer units.
Dependencies are inferred from the claim text.