Oligonucleotide for detecting Salmonella and method of detecting Salmonella

A process of amplifying Salmonella gene invA mRNA, wherein a specific sequence of Salmonella gene invA mRNA present in a sample is used as a template for synthesis of a cDNA employing an RNA-dependent DNA polymerase, the RNA of the formed RNA/DNA hybrid is digested by Ribonuclease H to produce a single-stranded DNA, said single-stranded DNA is then used as a template for production of a double-stranded DNA having a promoter sequence capable of transcribing RNA comprising said specific sequence or a sequence complementary to said specific sequence employing a DNA-dependent DNA polymerase, said double-stranded DNA produces an RNA transcription product in the presence of an RNA polymerase, and said RNA transcription product is then used as a template for cDNA synthesis employing said RNA-dependent DNA polymerase, the amplification process being characterized by employing a first oligonucleotide capable of specifically binding to Salmonella gene invA mRNA and comprising at least 10 contiguous bases of any of the sequences listed as SEQ. ID. Nos. 1 to 12 and a second oligonucleotide comprising at least 10 contiguous bases of any of the sequences listed as SEQ. ID. Nos. 19 to 23 and having a sequence homologous to a portion of the Salmonella gene invA mRNA sequence to be amplified, where either said first or second oligonucleotide includes said RNA polymerase promoter sequence at the 5′ end.

A process of amplifying Salmonella gene stn mRNA, wherein a specific sequence of Salmonella gene stn mRNA present in a sample is used as a template for synthesis of a cDNA employing an RNA-dependent DNA polymerase, the RNA of the formed RNA/DNA hybrid is digested by Ribonuclease H to produce a single-stranded DNA, said single-stranded DNA is then used as a template for production of a double-stranded DNA having a promoter sequence capable of transcribing RNA comprising said specific sequence or a sequence complementary to said specific sequence employing a DNA-dependent DNA polymerase, said double-stranded DNA produces an RNA transcription product in the presence of an RNA polymerase, and said RNA transcription product is then used as a template for cDNA synthesis employing said RNA-dependent DNA polymerase, the amplification process being characterized by employing a first oligonucleotide capable of specifically binding to Salmonella gene stn mRNA and comprising at least 10 contiguous bases of any of the sequences listed as SEQ. ID. Nos. 13 to 18 and a second oligonucleotide comprising at least 10 contiguous bases of any of the sequences listed as SEQ. ID. Nos. 24 to 27 and having a sequence homologous to a portion of the Salmonella gene stn mRNA sequence to be amplified, where either said first or second oligonucleotide includes the RNA polymerase promoter sequence at the 5′ end.