Method for detecting Escherichia coli
AssigneeDr. Chip Biotechnology Inc.
InventorsLu-Yieng Liu, Te-Yu Chung, Harn-Jing Terng
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Abstract
Specific nucleic acid sequences, e.g., SEQ ID NOs: 1-8, for detecting Escherichia coli. Also disclosed is a method of detecting Escherichia coli. The method includes providing a sample having a nucleic acid from an unknown microorganism; amplifying the nucleic acid with an upstream primer containing SEQ ID NO: 1 or 3 and a downstream primer containing SEQ ID NO: 2 or 4, each primer being 18-40 nucleotides in length; and detecting an amplification product. Detection of the amplification product, e.g., using SEQ ID NO: 5, 6, 7, or 8 as a probe, indicates the presence of Escherichia Coli.
Claims35
A set of nucleic acids comprising:
- a first nucleic acid containing SEQ ID NO:
- 1 or 3, and
- a second nucleic acid containing SEQ ID NO:
- 2 or 4,
- wherein each nucleic acid is 18-40 nucleotides in length.
A nucleic acid obtained from amplification of an Escherichia coli nucleic acid template with an upstream primer containing SEQ ID NO:
- 1 or 3 and a downstream primer containing SEQ ID NO:
- 2 or 4, wherein each primer is 18-40 nucleotides in length.
A nucleic acid that is 26-1000 nucleotides in length comprising a sequence selected from the group consisting of SEQ ID NOs:
- 5-8, or a sequence complementary thereto.
A method of detecting Escherichia coli, comprising:
- providing a sample having a nucleic acid from an unknown microorganism;
- amplifying the nucleic acid with an upstream primer containing SEQ ID NO:
- 1 or 3 and a downstream primer containing SEQ ID NO:
- 2 or 4, each primer being 18-40 nucleotides in length;
- and
- detecting an amplification product;
- whereby detection of the amplification product indicates the presence of Escherichia coli.
Dependencies are inferred from the claim text.