Alpha-2 adrenergic receptor polymorphisms
AssigneeUniversity of Cincinnati
InventorsKersten M. Small, Stephen B. Liggett
Life of this patent
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Abstract
The present invention includes polymorphisms in nucleic acids encoding the alpha-2B, alpha-2A, and alpha-2C adrenergic receptor and expressed alpha-2B, alpha-2A and alpha-2C adrenergic receptor molecule. The invention also pertains to methods and molecules for detecting such polymorphisms. The invention further pertains to the use of such molecules and methods in the diagnosis, prognosis, and treatment of diseases such as cardiovascular and central nervous system disease.
Claims17
An isolated polynucleotide encoding an alpha-2C adrenergic receptor molecule or fragment or complement of the polynucleotide, having a polymorphic site comprising ggggcggctgag (SEQ ID NO:
- 43) at nucleotide positions 964 to 975.
An isolated oligonucleotide that specifically hybridizes to a region of a polynucleotide encoding an alpha-2C adrenergic receptor molecule or fragment of the polynucleotide, wherein the region comprises at least one polymorphic site comprising ggggcggctgag (SEQ ID NO:
- 43) at nucleotide positions 964 to 975.
A method of genotyping an alpha-2C-adrenergic receptor gene comprising:
- (a) obtaining a sample having a polynucleotide encoding an alpha-2C-adrenergic receptor molecule or fragment or complement of the polynucleotide;
- and
- (b) detecting in the sample a polymorphic site comprising SEQ ID NO:
- 41 or SEQ ID NO:
- 43 at nucleotide positions 964 to 975 of the polynucleotide or fragment or complement of the polynucleotide.
A method of detecting a polymorphic site in a sample to determine alpha-2C-adrenergic receptor function, comprising:
- (a) obtaining the sample having a polynucleotide encoding an alpha-2C-adrenergic receptor molecule or fragment or complement of the polynucleotide;
- and
- (b) detecting in the sample a polymorphic site comprising SEQ ID NO:
- 41 or SEQ ID NO:
- 43 at nucleotide positions 964 to 975 of the polynucleotide or fragment or complement of the polynucleotide.
A method of predicting an individual's response to an agonist or antagonist, comprising:
- (a) obtaining a sample having a polynucleotide encoding an alpha-2C-adrenergic receptor molecule or fragment or complement of the polynucleotide from the individual;
- (b) detecting in the sample a polymorphic site comprising SEQ ID NO:
- 41 or SEQ ID NO:
- 43 at nucleotide positions 964 to 975 of the polynucleotide or fragment or complement of the polynucleotide;
- and
- (c) correlating the polymorphic site to a predetermined response thereby predicting the individual's response to the agonist or antagonist.
Dependencies are inferred from the claim text.