Detection of fusarium species infecting corn using the polymerase chain reaction
AssigneeSyngenta Participations AG
InventorsJames Joseph Beck, Charles Jason Barnett
Life of this patent
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Abstract
The present invention relates to the use of primers in polymerase chain reaction assays for the detection of a Fusarium proliferatum, F. verticillioides and F. subglutinans. Specific primers are identified as being useful for the identification of fungal isolates using PCR based techniques.
Claims18
A method for the detection of a fungal pathogen, comprising the steps of:
- (a) isolating DNA from a plant leaf infected with a pathogen;
- (b) subjecting said DNA to polymerase chain reaction amplification using at least one primer having sequence identity with at least 10 contiguous nucleotides of an Internal Transcribed Spacer sequence of a Fusarium spp.;
- and
- (c) detecting said fungal pathogen by visualizing the product or products of said polymerase chain reaction amplification.
A method for the detection of a fungal pathogen, comprising the steps of:
- (a) isolating DNA from a plant leaf infected with a pathogen;
- (b) subjecting said DNA to polymerase chain reaction amplification using at least one primer having sequence identity with at least 10 contiguous nucleotides of a mitochondrial small subunit rDNA gene from a Fusarium spp.;
- and
- (c) detecting said fungal pathogen by visualizing the product or products of said polymerase chain reaction amplification.
A diagnostic kit used in detecting a fungal pathogen comprising at least one primer having at least 10 contiguous nucleotides of an Internal Transcribed Spacer sequence of a Fusarium spp.
A diagnostic kit used in detecting a fungal pathogen comprising at least one primer comprising the sequence of SEQ ID NO:
- 9-12, 21 or 22.
A diagnostic kit used in detecting a fungal pathogen comprising at least one primer having at least 10 contiguous nucleotides of a mitochondrial small subunit rDNA gene of a Fusarium spp.
A diagnostic kit used in detecting a fungal pathogen comprising at least one primer having the nucleotide sequence of SEQ ID NO:
- 13-20, 23 or 24.
A diagnostic kit used in detecting a fungal pathogen comprising a pair of primers of
- a) SEQ ID NO:
- 15 and SEQ ID NO:
- 16;
- b) SEQ ID NO:
- 14 and SEQ ID NO:
- 18;
- c) SEQ ID NO:
- 14 and SEQ ID NO:
- 19;
- or
- d) SEQ ID NO:
- 14 and SEQ ID NO:
- 20.
Dependencies are inferred from the claim text.