Method for cloning polyketide synthase genes
OwnerDaniel Santiinventor
Life of this patent
Estimating the term…
Abstract
A method for obtaining “perfect probes” for type I modular polyketide synthase (PKS) or non-ribosomal peptide synthase (NRPS) gene clusters enables the identification of all such gene clusters in a genome. By sequencing small fragments of a random genomic DNA library containing one or more modular PKS or NRPS gene clusters, and identifying which fragments emanate from PKS or NRPS genes and knowing the approximate sizes of the genome and the target gene cluster, one can predict the frequency that a PKS or NRPS gene fragment will be present in the library sequenced.
Claims15
A method for generating a perfect probe for any PKS or NRPS gene or gene cluster in an organism, the method comprising the steps of:
- (a) generating a genomic library of vectors containing insert DNA from said organism;
- (b) generating nucleotide sequence information from said vectors;
- (c) comparing said nucleotide sequence information generated with sequence information from a known PKS or NRPS gene;
- and
- (d) identifying vectors with insert DNA that contains nucleotide sequences from a PKS or NRPS gene,
- wherein said insert DNA that contains nucleotide sequences from a PKS or NRPS gene is a perfect probe for said PKS or NRPS gene.
Dependencies are inferred from the claim text.