Use of defibrinated blood for manufacture of a hemoglobin-based oxygen carrier
AssigneeBiopure Corporation
InventorsMaria S. Gawryl, Robert A. Houtchens, William R. Light
Life of this patent
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Abstract
Red blood cells are purified by defibrinating whole blood and then filtering the defibrinated whole blood, whereby at least a portion of a plasma component is separated from the red blood cells to form a suspension of red blood cells, thereby purifying the red blood cells. Whole blood is defibrinated by, for example, using a chemical coagulating agent or mechanical agitation. Separation of the plasma component from red blood cells can be completed by, for example, diafiltration. The suspension of red blood cells can then be employed to produce a hemoglobin-based oxygen carrier.
Claims27
A method for preparing a hemoglobin solution, comprising the steps of:
- a) defibrinating whole blood;
- b) separating red blood cells from the defibrinated whole blood;
- and
- c) isolating hemoglobin molecules from the red blood cells, whereby a hemoglobin solution is formed.
The method of claim 13, further including the step of deoxygenating the hemoglobin solution.
A method for preparing a hemoglobin-based oxygen carrier, comprising the steps of:
- a) defibrinating whole blood;
- b) separating red blood cells from the defibrinated whole blood;
- c) isolating hemoglobin molecules of said cells to form a hemoglobin solution;
- and
- d) stabilizing the hemoglobin, thereby preparing the hemoglobin-based oxygen carrier.
The method of using defibrinated blood to manufacture a hemoglobin-based oxygen carrier, comprising the steps of:
- a) using collagen to defibrinate whole blood, thereby forming defibrinated whole blood;
- b) diafiltering the defibrinated whole blood;
- c) lysing the red blood cells by centrifugation to release hemoglobin, thereby forming hemoglobin solution;
- d) deoxygenating the hemoglobin solution;
- e) cross-linking the hemoglobin of the deoxygenated hemoglobin solution with glutaraldehyde, thereby producing a hemoglobin-based oxygen carrier;
- and
- f) preserving the hemoglobin-based oxygen carrier in an oxygen barrier primary package.
Dependencies are inferred from the claim text.