Methods for inactivation of pathogens in biological materials
AssigneeCerus Corporation
InventorsAdonis Stassinopoulos, David Cook
Life of this patent
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Abstract
Methods are provided for inactivation of pathogens in biomaterials. Pathogen inactivating agents are added to and mixed with biomaterials in an additive solution that is low in chloride and/or hypotonic, resulting in substantial increases in inactivation of bacterial pathogens, particularly Gram negative bacterial pathogens.
Claims67
An aqueous mixture for inactivation of bacteria comprising:
- (i) an additive solution wherein chloride ion, if present, is at a concentration of less than about 10 mM,
- (ii) a biological material suspected of containing the bacteria;
- and
- (iii) a pathogen inactivation compound in an amount sufficient to inactivate at least 1 log of the bacteria.
A method of inactivating a bacteria in a biological material suspected of containing the bacteria comprising:
- (i) contacting the biological material with an additive solution comprising a chloride concentration of less than about 10 mM,
- (ii) contacting the biological material with a pathogen inactivation compound in an amount sufficient to inactivate at least 1 log of the bacteria, and
- (iii) incubating the biological material contacted with the additive solution and the pathogen inactivation compound for sufficient time to inactivate at least 1 log of the bacteria.
A method of inactivating a bacteria in a biological material suspected of containing the bacteria comprising:
- (i) contacting the biological material with a first additive solution which is essentially chloride free,
- (ii) contacting the biological material with a pathogen inactivation compound in an amount sufficient to inactivate at least 1 log of the bacteria, wherein the pathogen inactivation compound has a greater inactivation efficiency against Yersinia enterocolitica when used with said first additive solution than when used with a second additive solution, said second additive solution comprising at least about 10 mM chloride ion;
- and
- (iii) incubating the biological material contacted with the first additive solution and the pathogen inactivation compound for sufficient time to inactivate at least 1 log of the bacteria.
A method of inactivating a bacteria in a biological material suspected of containing the bacteria comprising:
- (i) contacting the biological material with a pathogen inactivation compound in an amount sufficient to inactivate at least 1 log of the bacteria and an additive solution comprising a chloride concentration of less than about 10 mM, and
- (ii) incubating the biological material contacted with the additive solution and the pathogen inactivation compound for sufficient time to inactivate at least I log of the bacteria.
A method of inactivating a bacteria in a biological material suspected of containing the Gram negative bacteria comprising:
- (i) contacting the biological material with a first additive solution which is essentially chloride free and a pathogen inactivation compound in an amount sufficient to inactivate at least 1 log of the bacteria, wherein the pathogen inactivation compound has a greater inactivation efficiency against Yersinia enterocolitica when used with said first additive solution than when used with a second additive solution, said second additive solution comprising at least about 10 mM chloride ion;
- and
- (iii) incubating the biological material contacted with the first additive solution and the pathogen inactivation compound for sufficient time to inactivate at least 1 log of the bacteria.
A method of inactivating a bacteria in a biological material suspected of containing the bacteria comprising:
- (i) contacting the biological material with an additive solution that is essentially free of chloride ions and comprises about 26.6 mM sodium citrate, about 17 mM disodium phosphate, about 4.7 mM monosodium phosphate, about 1.6 mM adenine and about 42.5 mM mannitol,
- (ii) contacting the biological material with a pathogen inactivation compound in an amount sufficient to inactivate at least 1 log of the bacteria, and
- (iii) incubating the biological material contacted with the additive solution and the pathogen inactivation compound for sufficient time to inactivate at least 1 log of the bacteria.
Dependencies are inferred from the claim text.