Method of stabilizing a hydrophobin-containing solution and a method of coating a surface with a hydrophobin
OwnerMarcel Leo de Vocht, Hermann Abel B Wösten +2inventors
InventorsMarcel Leo de Vocht, Hermann Abel B Wösten, George Thomas Robillard, Joseph Gerard H Wessels
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Abstract
The invention relates to a method of stabilizing a hydrophobin-containing solution. According to the present invention, the hydrophobin is subjected to a treatment with a disulphide bridge-cleaving agent to yield unfolded hydrophobin, said treatment involving the prevention of the formation of disulphide bridges from cleaved disulphide bridges. The invention also relates to a method for coating a surface with a stabilized hydrophobin according to the present invention.
Claims7
Method of stabilizing a hydrophobin-containing solution, characterized in that the hydrophobin is subjected to a treatment with a disulphide bridge-cleaving agent to yield unfolded hydrophobin, said treatment involving the prevention of the formation of disulphide bridges from cleaved disulphide bridges, said treatment being chosen from the group consisting of i) a treatment with sulphite resulting in a modified hydrophobin carrying sulphite groups;
- ii) a treatment involving the prevention of the formation of disulphide bridges which comprises reacting hydrophobin reduced with a reducing agent with a sulfhydryl-protecting agent chosen to allow for removal of the sulfhydryl-protecting group formed yielding hydrophobin having removable sulfhydryl-protecting groups;
- and iii) a treatment involving the prevention of the formation of disulphide bridges which comprises exposing hydrophobin reduced with a reducing agent to an environment in which substantially no oxidizing agent is present.
Dependencies are inferred from the claim text.