Proteinaceous coating
AssigneeIsoTis N.V.
InventorsYuelian Liu, Klaas de Groot, Pierre Jean F. Layrolle
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Abstract
The invention relates to a method for providing a proteinaceous coating on a medical implant, comprising the steps of: submersing the implant in a first aqueous solution comprising a protein and magnesium, calcium and phosphate ions through which a gaseous weak acid is passed; degassing the solution; allowing a coating to precipitate onto the implant; submersing the coated implant in a second solution to redissolve the magnesium, calcium and phosphate ions and to obtain the proteinaceous coating.
Claims21
A method for providing a proteinaceous coating on a medical implant, comprising the steps of:
- submersing the implant in a first aqueous solution comprising a protein and magnesium, calcium and phosphate ions through which a gaseous weak acid is passed;
- degassing the solution;
- allowing a coating to precipitate onto the implant;
- submersing the coated implant in a second solution to redissolve the magnesium, calcium and phosphate ions and to obtain the proteinaceous coating.
A method according to any of the preceding claims, wherein the calcium and phosphate ions are present in the first solution in a molar ratio of between 1 and 3, preferably between 1.5 and 2.5.
A method according to any of the preceding claims, wherein the first solution comprises 0.1-20, preferably 1.5-10 mM magnesium ions.
A method according to any of the preceding claims, wherein the first solution further comprises 0-50 mM, preferably 0-42 mM carbonate ions.
A method according to any of the preceding claims, wherein the ionic strength of the first solution is in the range of 0.1-2 M, preferably of 0.15-1.5 M.
A method according to any of the preceding claims, wherein the pressure of the gaseous weak acid is in the range of 0.1-10 bar, preferably of 0.5-1.5 bar.
A method according to any of the preceding claims, wherein the temperature of the first and second solutions are independently chosen in the range of 5 to 80° C., preferably of to 50° C.
A method according to any of the preceding claims, wherein the protein is chosen from the group of albumin, casein, gelatin, lysosime, fibronectin, fibrin, chitosan, polylysine, polyalanine, polycysteine, growth factors, and combinations thereof.
A method according to any of the preceding claims, wherein the protein is present in the first solution in a concentration of between 0.001 and 10 g/l.
A method according to any of the preceding claims, wherein the second solution is an aqueous acidic solution.
A method according to any of the preceding claims, wherein the second solution comprises a sequestering or complexing agent.
A medical implant comprising a proteinaceous coating obtainable by a method according to any of the preceding claims.
Dependencies are inferred from the claim text.