Immunogenic detoxified mutant e. coli lt-a toxin
OwnerMariagrazia Pizza, Marzia Monica Giuliani +1inventors
InventorsMARIAGRAZIA PIZZA, MARZIA MONICA GIULIANI, RINO RAPPUOLI
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Abstract
An immunogenic detoxified protein is provided which comprises the amino acid sequence of subunit A of an E. coli heat labile toxin (LT-A) or a fragment thereof in which at least amino acid Ala-72 of the A subunit is mutated, preferably by substitution with Arg. The toxoid is useful as vaccine against an enterotoxigenic strain of E. coli and is produced by recombinant DNA means by site-directed mutagenesis. It is also an effective adjuvant.
Claims6
An immunogenic detoxified protein comprising the amino acid sequence of subunit A of an E.coli heat labile toxin (LT-A), or a fragment thereof, for use as a mucosal adjuvant, wherein amino acid Ala-72 in said sequence or fragment is substituted with an arginine residue.
An immunogenic composition comprising (i) a pharmaceutically acceptable carrier and (ii) an immunogenic detoxified protein comprising the amino acid sequence of subunit A of an E.coli heat labile toxin (LT-A), or a fragment thereof, for use as a vaccine, wherein amino acid Ala-72 in said sequence or fragment is substituted with an arginine residue.
The use of an immunogenic detoxified protein comprising the amino acid sequence of subunit A of an E.coli heat labile toxin (LT-A), or a fragment thereof, in the manufacture of a vaccine, wherein amino acid Ala-72 in said sequence or fragment is substituted with an arginine residue.
A method of vaccinating a mammal, comprising administering (i) an effective amount of an immunogenic detoxified protein comprising the amino acid sequence of subunit A of an E.coli heat labile toxin (LT-A), or a fragment thereof, wherein amino acid Ala-72 in said sequence or fragment is substituted with an arginine residue, optionally in association with (ii) a second immunogenic antigen.
Dependencies are inferred from the claim text.