Human glucose-dependent insulin-secreting cell line
AssigneeCedars-Sinai Medical Center
InventorRiccardo Perfetti
Life of this patent
Estimating the term…
Abstract
Disclosed herein is a novel cell line of human pancreatic cells that secrete insulin in a glucose-dependent manner. The cell line comprises pancreatic cells, such as PANC-1 cells, which are transfected so as to express IDX-1 and cultured in GLP-1. The cell line may be used to investigate the function and development of pancreatic cells, as well as to test the efficacy of drugs that stimulate insulin secretion.
Claims55
An insulin-secreting cell, comprising a pancreatic cell transfected with IDX-1 cDNA and cultured in glucogon-like peptide-1 (GLP-1).
A cell that exhibits a dose-dependent response of insulin secretion when exposed to glucose, the cell comprising a pancreatic cell transfected with IDX-1 cDNA and cultured in glucogon-like peptide-1.
A method of producing insulin-secreting cells, the method comprising the steps of
- providing a line of pancreatic cells;
- transfecting the cells with genetic material selected from the group consisting of IDX-1 DNA, IDX-1 cDNA, and IDX-1 mRNA;
- and
- culturing the cells in glucogon-like peptide-1 (GLP-1).
A method of determining the ability of a drug to stimulate cells to secrete insulin, the method comprising the steps of
- providing pancreatic cells transfected with IDX-1 cDNA and cultured in GLP-1;
- exposing the cells to the drug;
- and
- measuring the amount of insulin secreted by the cells.
Dependencies are inferred from the claim text.