Use of bacterial phage associated lysing proteins for the prophylactic and therapeutic treatment of various illnesses
OwnerVincent Fischetti, LAwrance Loomis +1inventors
InventorsVincent Fischetti, LAwrance Loomis, David Trudil
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Abstract
A composition and method for treating bacterial infections is disclosed which comprises the treatment of an individual with an effective amount of at least one lytic protein, peptide, or peptide fragment thereof, wherein said at least one lytic protein or peptides is in a natural or modified form The composition further discloses several carrier compositions suitable for site-specific delivery of the composition. Further disclosed are the method of use of the composition of the invention. These methods include therapeutic, diagnostic, prognostic and drug screening methods.
Claims28
A pharmaceutical composition comprising an effective amount of at least one phage lytic and/or holin protein, or peptides , and a pharmaceutically acceptable carrier.
A method for treating, preventing or ameliorating bacterial infections at a mucosal surface comprising the steps:
- a) obtaining a composition comprising an effective amount of at least one lytic protein peptides or peptide fragments thereof;
- and
- b) applying said composition to the mucosal surface,
- wherein the lytic protein, peptides, or peptide fragments thereof is produced by infecting a bacterium causing said infection with a bacteriophage specific for said bacteria and wherein said bacteria produces said at least one recombinant lytic protein selected from the group consisting of chimeric lytic proteins, shuffled lytic proteins, and combinations thereof.
A method for detecting the presence of a gene or a gene fragment encoding a lytic protein or a peptide in a sample, comprising the steps of:
- a) contacting the sample with a nucleic acid probe or primer which selectively hybridizes to gene or gene fragment ;
- and
- b) determining whether the nucleic acid probe or primer binds to the gene or gene fragment in the sample.
A kit comprising a compound which selectively hybridizes to gene or gene fragment encoding a lytic protein, or peptide and instructions for use.
Dependencies are inferred from the claim text.