Systems and methods for detecting a particle
OwnerEdgar A. Arriaga, Ciaran F. Duffy +1inventors
InventorsEdgar A. Arriaga, Ciaran F. Duffy, Dmitry Andreyev
Life of this patent
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Abstract
Systems and methods for detecting particles are provided. In one embodiment, capillary electrophoresis is used to separate particles that may be detected by methods including, for example, laser induced fluorescence. The systems and methods are useful for separating and evaluating individual particles including, for example, subcellular particles.
Claims43
A method of detecting a particle comprising:
- providing a sample comprising a plurality of particles;
- applying an electric field to separate a particle;
- generating a signal characteristic of the separated particle;
- sampling the signal at a sampling rate effective to detect the separated particle;
- and
- providing output based on the sampled signal that is characteristic of the detected separated particle.
A method of detecting a particle comprising:
- providing a sample comprising a plurality of particles;
- applying an electric field to separate a particle;
- generating a signal characteristic of the separated particle;
- sampling the signal at a rate of at least about 40 cycles per second to detect the separated particle;
- and
- providing output based on the sampled signal that is characteristic of the detected separated particle.
A method of detecting a particle comprising:
- providing a defined sample volume comprising a plurality of particles;
- directing the particles through a separation device;
- allowing the particles to interact with an inner surface of the separation device to separate a particle;
- generating a signal characteristic of the separated particle;
- sampling the signal at a sampling rate effective to detect the separated particle;
- and
- providing output based on the sampled signal that is characteristic of the detected separated particle.
A method of detecting a particle comprising:
- providing a defined sample volume comprising a plurality of particles;
- separating a particle;
- generating a signal characteristic of the separated particle;
- sampling the signal at a rate of at least about 40 cycles per second to detect the separated particle;
- and
- providing output based on the sampled signal that is characteristic of the detected separated particle.
A method of detecting a particle comprising:
- providing a defined sample volume comprising a particle;
- applying an electric field to displace the particle based on an electrophoretic property of the particle;
- and
- providing output characteristic of the displaced particle to detect the displaced particle.
A method of detecting a plurality of particles comprising:
- providing a sample comprising a plurality of particles;
- directing the particles through a separation device to provide a plurality of separated particles;
- generating a signal characteristic of the separated particles;
- sampling the signal at a sampling rate effective to detect at least about 50% of the separated particles;
- and
- providing output based on the sampled signal that is characteristic of the separated detected particles.
A system for detecting a particle comprising:
- a separation device operable to receive a defined sample volume comprising a plurality of particles;
- an electric field application device operable to apply an electric field across at least a portion of the sample volume to separate a particle;
- a signal generating device operable to generate a signal characteristic of the separated particle;
- and
- an output device operable to sample the signal at a rate effective to detect the separated particle and to provide output based on the sampled signal that is characteristic of the detected separated particle.
A system for detecting a particle comprising:
- a separation device operable to receive a sample comprising a plurality of particles;
- an electric field application device operable to apply an electric field across at least a portion of the sample to separate a particle;
- a signal generating device operable to generate a signal characteristic of the separated particle;
- and
- an output device operable to sample the signal at a rate of at least about 40 cycles per second to detect the separated particle and to provide output based on the sampled signal that is characteristic of the detected separated particle.
A system for detecting a particle comprising:
- a separation device comprising a defined sample volume comprising a plurality of particles, wherein the separation device has an inner surface that interacts with the particles;
- a device operable to direct the particles through the separation device to separate a particle;
- a signal generating device operable to generate a signal characteristic of the separated particle;
- and
- an output device operable to sample the signal at a rate of at least about 40 cycles per second to detect the separated particle and to provide output based on the sampled signal that is characteristic of the detected separated particle.
A system for detecting a separated particle provided in a separation device, wherein the separation device is operable to receive a defined sample volume comprising a plurality of particles, the system comprising:
- a signal generating device operable to generate a signal characteristic of the separated particle;
- and
- an output device operable to sample the signal at a rate of at least about 40 cycles per second to detect the separated particle and to provide output based on the sampled signal that is characteristic of the detected separated particle.
A method of detecting a particle using a system for detecting a separated particle provided in a separation device, wherein the separation device is operable to receive a defined sample volume comprising a plurality of particles, the method comprising:
- generating a signal characteristic of the separated particle;
- sampling the signal at a rate of at least about 40 cycles per second to detect the separated particle;
- and
- providing output based on the sampled signal that is characteristic of the detected separated particle.
Dependencies are inferred from the claim text.