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AssigneeAventis Pharma S.A.
InventorsHerman Slijkhuis, Eric Bastiaan Smaal, Gerardus Cornelis Maria Selten
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An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting a of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17α-hydroxy-progesterone; the conversion of 17α-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in said host.
An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of:
A recombinant host cell and progeny thereof comprising cells of micro-organisms, plants or animals containing, besides proper control sequences, heterologous DNA coding for proteins either from the group comprising proteins which are functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step isselected from the group consisting of:
In a process for the preparation of hydrocortisone from sterols, comprising culturing a recombinant cell in a nutrient medium, the improvement comprising a recombinant host containing an expression c assette, operable in a rec ombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing at least one oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of:
Dependencies are inferred from the claim text.