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AssigneeThe UAB Research Foundation
InventorsLaurence Andrew Ball, Kyle Leslie Johnson, Karyn Nicole Johnson, B. Duane Price
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The present invention describes the production of a nodavirus-based DNA vector that drives abundant expression of foreign genes in a wide variety of cell types. The DNA plasmid is initially transcribed by a host-cell RNA polymerase to produce primary transcripts from which a nodaviral RNA-dependent RNA polymerase (RNA replicase) is translated. These primary transcripts are then amplified by the RNA replicase in an autonomous, cytoplasmic RNA replication. Such a vector is a useful addition to the current arsenal of expression vectors, and well suited to laboratory-scale and larger-scale expression of transcripts and/or proteins in eukaryotic cells.
An isolated cDNA encoding a Nodamura virus RNA1 genome segment and having the sequence shown in SEQ ID No. 1.
An isolated cDNA encoding a Nodamura virus RNA2 genome segment and having the sequence shown in SEQ ID No. 2.
An isolated cDNA encoding a Pariacoto virus RNA1 genome segment and having the sequence shown in SEQ ID No. 3.
An isolated cDNA encoding a Pariacoto virus RNA2 genome segment and having the sequence shown in SEQ ID No. 4.
A nodavirus-based DNA expression vector, wherein said expression vector comprises:
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