Vascular tissue preferred promoters
AssigneePioneer Hi-Bred International, Inc.
InventorsShane E. Abbitt, Chun Ping Li, Xiaomu Niu
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Abstract
The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a vascular tissue-preferred promoter for the gene encoding prunasin hydrolase and sequences isolated therefrom. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the vascular tissue-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
Claims24
An isolated nucleic acid molecule having a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO.:
- 1 or SEQ ID NO.:
- 2;
- b) a nucleotide sequence comprising at least 50 contiguous nucleotides of the sequence set forth in SEQ ID NO.:
- 1 or SEQ ID NO.:
- 2;
- c) a nucleotide sequence comprising a sequence having at least 70% identity to the sequence set forth in SEQ ID NO.:
- 1 or SEQ ID NO.:
- 2, or a fragment thereof, wherein said sequence regulates transcription;
- and,
- d) a nucleotide sequence that hybridizes under stringent conditions to sequence of a), b), c), or a complement thereof.
An isolated promoter that is capable of driving transcription in a vascular tissue-preferred manner, wherein the promoter is natively associated with DNA coding for Prunus serotina prunasin hydrolase.
A plant having stably incorporated into its genome a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO.:1 or SEQ ID NO.:2;
- b) a nucleotide sequence comprising at least 50 contiguous nucleotides of the sequence set forth in SEQ ID NO.:1 or SEQ ID NO.:
- 2;
- c) a nucleotide sequence comprising a sequence having at least 70% identity to the sequence set forth in SEQ ID NO.:1 or SEQ ID NO.:
- 2, or a fragment thereof, wherein said sequence regulates transcription;
- and,
- d) a nucleotide sequence that hybridizes under stringent conditions to sequence of a), b), c), or a complement thereof.
A method for expressing a heterologous nucleotide sequence in a plant, said method comprising stably integrating into a plant cell a heterologous nucleotide sequence of interest operably linked to a promoter, wherein the promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO.:
- 1 or SEQ ID NO.:
- 2;
- b) a nucleotide sequence comprising at least 100 contiguous nucleotides of the sequence set forth in SEQ ID NO.:
- 1 or SEQ ID NO.:
- 2;
- c) a nucleotide sequence comprising a sequence having at least 70% identity to the sequence set forth in SEQ ID NO.:
- 1 or SEQ ID NO.:
- 2, or a fragment thereof, wherein said sequence regulates transcription;
- and,
- d) a nucleotide sequence that hybridizes under stringent conditions to the complement of a sequence of a), b), c), or a complement thereof.
Dependencies are inferred from the claim text.