Adenoviruses for control of gene expression
AssigneeAdVec, Inc.
InventorsFrank L. Graham, Martina Anton, Silvia Bacchetti, Ping Wang, Michael A. Rudnicki, William J. Muller
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Abstract
The invention claims a class of adenovirus vectors for delivering recombinases to a large number of cells of different origins, and methods for regulating the expression of a gene in transfected mammalian cells in culture and in cells of transgenic animals, comprising infecting said cells with an Ad vector encoding a recombinase whose target site is present at or adjacent to the gene, wherein the action of the recombinase regulates the expression of said gene.
Claims17
An adenovirus comprising a gene, the expression of which is under the control of a site-specific recombinase.
An adenovirus comprising a gene, and site-specific recombinase target sites operably linked to the gene, whereby recombination between said target sites mediated by a site-specific recombinase alters expression of the gene.
An adenovirus comprising a gene, and site-specific recombinase target sites flanking a promoter sequence that promotes expression of the gene, whereby recombination between said target sites mediated by a site-specific recombinase removes the promoter sequence, resulting in decreased expression of the gene.
An adenovirus comprising a gene, a promoter sequence directed away from said gene, and two site specific recombinase target sites flanking said promoter but oriented in opposite orientation to one another, whereby recombination between said target sites mediated by a site specific recombinase inverts the promoter sequence, resulting in increased expression of the gene.
An adenovirus comprising a gene, and site-specific recombinase target sites flanking a DNA spacer sequence located between a promoter sequence and the gene, whereby recombination between said target sites mediated by a site-specific recombinase removes the DNA spacer sequence, resulting in increased expression of the gene.
An adenovirus comprising a gene and site-specific recombinase target sites flanking a coding sequence for the gene, whereby recombination between said target sites mediated by a site-specific recombinase removes the coding sequence, resulting in decreased expression of the gene.
An adenovirus comprising a gene, a portion of said gene comprising a coding sequence oriented in an opposite direction to normal translation of the gene, and two site specific recombinase target sites flanking said coding sequence but oriented in opposite orientation to one another, whereby recombination between said target sites mediated by a site specific recombinase inverts the coding sequence, resulting in increased expression of the gene.
An adenovirus comprising a gene and site-specific recombinase target sites flanking the gene, whereby recombination between said target sites mediated by a site-specific recombinase removes the gene, resulting in decreased expression of the gene.
An adenovirus comprising a gene, said gene oriented in an opposite direction to normal translation of the gene, and two site specific recombinase target sites flanking said gene but oriented in opposite orientation to one another, whereby recombination between said target sites mediated by a site specific recombinase inverts the gene, resulting in increased expression of the gene.
Dependencies are inferred from the claim text.