Commercial use of arabidopsis for production of human and animal therapeutic and diagnostic proteins
OwnerNewell Bascomb, Mark Bossie +6inventors
InventorsNewell Bascomb, Mark Bossie, Marina Skarjinskaia, Lynne Hirayama, Gerald Hall, Thomas Petty II, Andrei Golovko, Melissa Campo
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Abstract
The invention provides methods that make it possible to take advantage of various growth parameters of Arabidopsis in order to grow dense populations of the plant in controlled indoor environments for the purpose of harvesting the biomass and isolating proteins, particularly recombinant proteins suitably for pharmaceutical applications.
Claims50
A method for producing large scale amounts of a recombinant protein in Arabidopsis, comprising:
- (a) introducing at least one expression cassette capable of expressing the recombinant protein into Arabidopsis cells;
- (b) identifying a cell which expresses a desired level and/or activity of the recombinant protein;
- (c) obtaining Arabidopsis seeds from progeny of the cell;
- (d) cultivating the seeds under conditions to produce seed rapidly;
- and
- (e) screening plants obtained from the seeds to identify plants which express a desired level and/or activity of recombinant protein;
- (f) cultivating at least two generations of the protein producing plants and selecting the highest protein producers under conditions to produce seeds rapidly;
- and
- (g) cultivating a plant line expressing the highest amount of protein, under conditions to produce at least about 50 grams of biomass per square foot.
A method for producing a recombinant protein in Arabidopsis, comprising:
- a. growing an Arabidopsis variety comprising at least one expression cassette for expressing a recombinant protein, under conditions that promote the production of vegetative and leafy biomass;
- b. harvesting at least a portion of the Arabidopsis containing recombinant protein prior to seed formation;
- and
- c. recovering at least one gram of recombinant protein in a one year period.
A biomass of Arabidopsis comprising at least about 10 grams, wherein at least 0.1% of the soluble protein of said Arabidopsis biomass comprises a recombinant protein.
A method of providing a protein to a human being comprising orally administering Arabidopsis cells or a fraction thereof to the human being.
Dependencies are inferred from the claim text.