Novel receptors
AssigneeTularik Inc.
InventorsDaniel Chi-Hong Lin, Jiagang Zhao, Jin-Long Chen, Gene Cutler
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Abstract
The invention provides isolated nucleic acid and amino acid sequences of novel receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of the receptors.
Claims67
An isolated nucleic acid encoding a G-protein coupled receptor polypeptide, the nucleic acid encoding a polypeptide comprising greater than 70% amino acid identity to an amino acid sequence of SEQ ID NO:6, SEQ ID NO:4, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, or SEQ ID NO:16.
An isolated nucleic acid encoding a G-protein coupled receptor polypeptide, wherein the nucleic acid specifically hybridizes under stringent hybridization conditions to a nucleic acid having a nucleotide sequence of SEQ ID NO:5, SEQ ID NO:3, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, or SEQ ID NO:15.
An isolated nucleic acid encoding a G-protein coupled receptor polypeptide, the polypeptide encoded by the nucleic acid comprising greater than about 70% amino acid identity to a polypeptide having an amino acid sequence of SEQ ID NO:6, SEQ ID NO:4, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, or SEQ ID NO:16, wherein the nucleic acid selectively hybridizes under moderately stringent hybridization conditions to a nucleotide sequence of SEQ ID NO:5, SEQ ID NO:3, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, or SEQ ID NO:15.
An isolated nucleic acid encoding a G-protein coupled receptor polypeptide, wherein the nucleic acid encodes a polypeptide comprising at least 25 contiguous amino acids of the amino acid sequence of SEQ ID NO:6, SEQ ID NO:4, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, or SEQ ID NO:16.
An isolated nucleic acid encoding a G-protein coupled receptor polypeptide, wherein the nucleic acid encodes a polypeptide comprising greater than 90% amino acid identity to an amino acid sequence of SEQ ID NO:2 or SEQ ID NO:14.
An isolated nucleic acid encoding a G-protein coupled receptor polypeptide, the polypeptide encoded by the nucleic acid comprising greater than about 90% amino acid identity to a polypeptide having an amino acid sequence of SEQ ID NO:2 or SEQ ID NO:14, wherein the nucleic acid selectively hybridizes under moderately stringent hybridization conditions to a nucleotide sequence of SEQ ID NO:1 or SEQ ID NO:13.
An isolated G-protein coupled receptor polypeptide, the polypeptide comprising greater than about 70% amino acid sequence identity to an amino acid sequence of SEQ ID NO:6, SEQ ID NO:4, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, or SEQ ID NO:16.
An isolated G-protein coupled receptor polypeptide, the polypeptide comprising greater than about 90% amino acid sequence identity to an amino acid sequence of SEQ ID NO:2 or SEQ ID NO:14.
A method for identifying a compound that modulates signal transduction, the method comprising:
- (i) contacting the compound with a polypeptide comprising greater than 70% amino acid sequence identity to the amino acid sequence of SEQ ID NO:6, SEQ ID NO:4, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, or SEQ ID NO:16;
- and
- (ii) determining the functional effect of the compound upon the polypeptide.
A method for identifying a compound that modulates signal transduction, the method comprising the steps of:
- (i) contacting the compound with a polypeptide comprising greater than 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:14;
- and
- (ii) determining the functional effect of the compound upon the polypeptide.
A method of detecting the presence of an TGR-GPCR or a EDG-GPCR nucleic acid or polypeptide in human tissue, the method comprising the steps of:
- (i) isolating a biological sample;
- (ii) contacting the biological sample with a TGR-GPCR-specific reagent or a EDG-GPCR-specific reagent that selectively associates with an TRG-GPCR nucleic acid or polypeptide or a EDG-GPCR nucleic acid or polypeptide;
- and,
- (iii) detecting the level of TGR-GPCR-specific reagent or EDG-GPCR-specific reagent that selectively associates with the sample.
Dependencies are inferred from the claim text.