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US 2003/0082811 A1Published application
Adenovirus E4 protein variants for virus production
AssigneeWake Forest University
InventorsJoseph S. Orlando, David A. Ornelles
Life of this patent
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AssigneeWake Forest University
InventorsJoseph S. Orlando, David A. Ornelles
Estimating the term…
A method of packaging a recombinant viral vector is carried out by: (a) providing a packaging cell, the packaging cell containing and expressing a nucleic acid encoding a mutant adenovirus E4orf6 protein, the E4orf6 protein containing at least one mutation that renders the protein non-toxic to the host cell; (b) transfecting or infecting the packaging cell with a nucleic acid that encodes a recombinant viral vector (e.g., an adenovirus vector or an adeno-associated virus vector), where the vector lacks a functional gene encoding E4orf6 protein; (c) culturing the transfected cells; and then (d) collecting packaged recombinant viral vector from the cultured cells. Nucleic acids, vectors and packaging cells used for carrying out the methods, as well as proteins utilized in the methods, are also described.
A method of packaging a recombinant viral vector, comprising the steps of:
A packaging cell, said packaging cell containing and expressing a nucleic acid encoding a mutant adenovirus E4orf6 protein, said E4orf protein containing at least one mutation in the region encoding amino acids 230 to 260 that renders said protein non-toxic to a host cell in which said protein is expressed.
A nucleic acid encoding a mutant adenovirus E4orf6 protein, said E4orf6 protein containing at least one mutation in the region encoding amino acids 230 to 260 that renders said protein non-toxic to a host cell in which said protein is expressed.
A mutant adenovirus E4orf6 protein, said E4orf6 protein containing at least one mutation in the region encoding amino acids 230 to 260 that that renders said protein non-toxic to a host cell in which said protein is expressed.
Dependencies are inferred from the claim text.