Transgenic mice containing TRP6 calcium ion channel gene disruptions
OwnerKeith D. Alleninventor
Life of this patent
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Abstract
The present invention relates to transgenic animals, as well as compositions and methods relating to the characterization of gene function. Specifically, the present invention provides transgenic mice comprising mutations in a TRP6 gene. Such transgenic mice are useful as models for disease and for identifying agents that modulate gene expression and gene function, and as potential treatments for various disease states and disease conditions.
Claims22
A targeting construct comprising:
- (a) a first polynucleotide sequence homologous to at least a first portion of a TRP6 gene;
- (b) a second polynucleotide sequence homologous to at least a second portion of the TRP6 gene;
- and
- (c) a selectable marker.
A method of producing a targeting construct, the method comprising:
- (a) providing a first polynucleotide sequence homologous to at least a first portion of a TRP6 gene;
- (b) providing a second polynucleotide sequence homologous to at least a second portion of the TRP6 gene;
- (c) providing a selectable marker;
- and
- (d) inserting the first sequence, second sequence, and selectable marker into a vector to produce the targeting construct.
A cell comprising a disruption in a TRP6 gene.
A non-human transgenic animal comprising a disruption in a TRP6 gene.
A method of identifying an agent that modulates the expression or function of a TRP6 gene, the method comprising:
- (a) providing a non-human transgenic animal comprising a disruption in the TRP6 gene;
- (b) administering the agent to the non-human transgenic animal;
- and
- (c) determining whether the expression or function of the disrupted TRP6 gene in the non-human transgenic animal is modulated.
A method of identifying an agent that modulates the expression or function of a TRP6 gene, the method comprising:
- (a) providing a cell comprising a disruption in the TRP6 gene;
- (b) contacting the cell with the agent;
- and
- (c) determining whether the expression or function of the TRP6 gene is modulated.
A transgenic mouse comprising a disruption in a TRP6 gene, wherein there is no significant expression of the TRP6 gene in the transgenic mouse.
A transgenic mouse comprising a disruption in a TRP6 gene, wherein the transgenic mouse exhibits an increased pain threshold, relative to wild-type control mice.
A method of identifying an agent that ameliorates a phenotype associated with a disruption in a TRP6 gene, the method comprising:
- (a) administering an agent to a transgenic mouse comprising a disruption in the TRP6 gene;
- and
- (b) determining whether the agent ameliorates the phenotype.
An agonist or antagonist of TRP6.
Phenotypic data associated with a transgenic mouse comprising a disruption in a TRP6 gene, wherein the phenotypic data is in an electronic database.
Dependencies are inferred from the claim text.