Factor VIII glycoforms
AssigneeLot 41 Acquisition Foundation, LLC
InventorsMyung-Sam Cho, Sham-Yuen Chan
Life of this patent
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Abstract
The invention concerns glycosylated proteins having human factor VIII activity. In a preferred embodiment, the protein is glycosylated with oligosaccharides that include an alpha-(2,6)-linked sialic acid and a bisecting GlcNAc linked to a core beta-mannose.
Claims46
An isolated glycosylated protein having factor VIII procoagulant activity comprising an N-linked oligosaccharide having an alpha-(2,6)-linked sialic acid.
An isolated glycosylated protein having factor VIII procoagulent activity comprising an N-linked oligosaccharide having a bisecting GlcNAc linked to a core beta-mannose.
A glycosylated protein having factor VIII procoagulant activity comprising the amino acid sequence of SEQ ID NO:
- 1 and an N-linked oligosaccharide having an alpha-(2,6)-linked sialic acid.
A glycosylated protein having factor VIII procoagulant activity comprising the amino acid sequence of SEQ ID NO:
- 1 and a bisecting GlcNAc linked to a core beta-mannose.
A glycosylated protein having a 90-kd heavy chain and an 80-kd light chain linked by a linker polypeptide of about 14 amino acids, said glycosylated protein having factor VIII procoagulent activity in humans and having an alpha-(2,6)-linked sialic acid.
A glycosylated protein having a 90-kd heavy chain and an 80-kd light chain linked by a linker polypeptide of about 14 amino acids, said glycosylated protein having factor VIII procoagulent activity in humans and having an N-linked oligosaccharide comprising a bisecting GlcNAc linked to a core beta-mannose.
A protein having Factor VIII procoagulent activity, wherein the amino acid sequence of the protein and the amino acid sequence of SEQ ID NO:
- 1 have at least 62% identity and the protein comprises an N-linked oligosaccharide comprising alpha-(2,6)-linked sialic acid.
A protein having factor VIII procoagulent activity, wherein the amino acid sequence of the protein and the amino acid sequence of SEQ ID NO:
- 1 have at least 62% identity and the protein comprises an N-linked oligosaccharide comprising a bisecting GlcNAc linked to a core beta-mannose.
Dependencies are inferred from the claim text.