Specific binding analysis method and device used therefor
AssigneeMatsushita Electric Industrial Co., Ltd.
InventorTatsurou Kawamura
Life of this patent
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Abstract
Disclosed is a specific binding analysis method capable of controlling the amount of specific binding to freely setting the sensitivity, concentration range and the like in an analysis, and a device used therefor. In order to optimize the amount of specific binding, the specific binding analysis method and the device used therefor restrain the amount and velocity of a sample passing, by capillarity, through a detection zone, by controlling the dimensions, ventilation resistance, hydrophilicity and the like in the portion of the device where the sample passes through.
Claims14
A specific binding analysis method for qualitatively or quantitatively analyzing an analyte in a sample, comprising the steps of:
- providing a specific binding analysis device comprising:
- a sample application zone for applying a sample containing an analyte;
- a space forming zone which is connected to said sample application zone and capable of exhibiting capillarity;
- and a detection zone which is provided in said space forming zone and capable of detecting a signal attributed to a specific binding reaction;
- applying said sample to said sample application zone to allow said sample to move, by capillarity, into said detection zone in said space forming zone to cause a specific binding reaction, thereby generating a signal attributed to said specific binding reaction, said signal being detected to perform a qualitative or quantitative analysis;
- and
- restraining a velocity at which said sample passes through said detection zone such that an intensity of said signal is substantially constant for samples having the same concentration, thereby controlling a time during which said specific binding reaction occurs.
A specific binding analysis device for performing a qualitative or quantitative analysis of an analyte contained in a sample, said device comprising:
- a sample application zone for applying a sample containing an analyte;
- a space forming zone which is connected to said sample application zone and capable of exhibiting capillarity;
- a detection zone which is provided in said space forming zone and capable of detecting a signal attributed to a specific binding reaction;
- and
- a ventilation resistance controlling means for restraining a velocity at which said sample passes through said detection zone,
- wherein a sample applied to said sample application zone moves, by capillarity, to said detection zone in said space forming zone to cause a specific binding reaction and a signal attributed to said specific binding reaction is detected to perform a qualitative or quantitative analysis.
Dependencies are inferred from the claim text.