Antibodies that specifically bind receptor
AssigneeUniversity of Wyoming
InventorLEE A. BULLA
Life of this patent
Estimating the term…
Abstract
The cDNA that encodes a glycoprotein receptor from the tobacco hornworm which binds a Bacillus thuringiensis toxin has been obtained and sequenced. The availability of this cDNA permits the retrieval of DNAs encoding homologous receptors in other insects and organisms as well as the design of assays for the cytotoxicity and binding affinity of potential pesticides and the development of methods to manipulate natural and/or introduced homologous receptors and, thus, to destroy target cells, tissues and/or organisms.
Claims15
A method to identify agents that bind to a BT-toxin receptor, said method comprising the steps of:
- i) contacting an agent with a BT-toxin binding receptor selected from the group consisting of a) a cell that has been altered to contain a nucleic acid molecule that encodes the amino acid sequence of SEQ ID No:2, b) a cell that has been altered to contain a nucleic acid molecule that encodes a fragment of the amino acid sequence of SEQ ID No:2 that binds to a BT toxin, c) a cell that has been altered to contain a nucleic acid molecule encoding a BT-toxin receptor that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency, d) a cell that has been altered to contain a nucleic acid molecule that encodes a fragment of a BT-toxin receptor that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency and that binds to a BT toxin, e) an isolated protein with an amino acid sequence of SEQ ID No:2, f) an isolated fragment of a protein with an amino acid sequence of SEQ ID No:2, said fragment containing a BT-toxin binding domain, g) an isolated BT-toxin receptor that is encoded by a nucleic acid molecule that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency, and h) an isolated fragment of a BT-toxin receptor that is encoded by a nucleic acid molecule that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency, and
- ii) determining whether said agent binds to said BT-toxin receptor.
A method to identify agents that block the binding of a BT-toxin to a BT-toxin receptor, said method comprising the steps of:
- i) contacting an agent, in the presence and absence of a BT-toxin, with a BT-toxin binding receptor selected from the group consisting of a) a cell that has been altered to contain a nucleic acid molecule that encodes the amino acid sequence of SEQ ID No:2, b) a cell that has been altered to contain a nucleic acid molecule that encodes a fragment of the amino acid sequence of SEQ ID No:2 that binds to a BT toxin, c) a cell that has been altered to contain a nucleic acid molecule encoding a BT-toxin receptor that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency, d) a cell that has been altered to contain a nucleic acid molecule that encodes a fragment of a BT-toxin receptor that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency and that binds to a BT toxin, e) an isolated protein with an amino acid sequence of SEQ ID No:2, f) an isolated fragment of a protein with an amino acid sequence of SEQ ID No:2, said fragment containing a BT-toxin binding domain, g) an isolated BT-toxin receptor that is encoded by a nucleic acid molecule that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency, and h) an isolated fragment of a BT-toxin receptor that is encoded by a nucleic acid molecule that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency, and
- ii) determining whether said agent blocks the binding of said BT-toxin to said BT-toxin receptor.
An isolated antibody, wherein said antibody binds to a protein selected from the group consisting of a) a BT-toxin receptor protein with an amino acid sequence of SEQ ID No:2, and b) a BT-toxin receptor protein that is encoded by a nucleic acid molecule that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency, or a fragment of said antibody, wherein said antibody fragment binds to said BT-toxin.
An isolated BT-toxin receptor protein selected from the group consisting of a) a BT-toxin receptor protein with an amino acid sequence of SEQ ID No:2, b) a BT-toxin receptor protein that is encoded by a nucleic acid molecule that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency, c) a fragment of a BT-toxin receptor protein with an amino acid sequence of SEQ ID No:2, said fragment being able to bind to a BT-toxin, and d) a fragment of a BT-toxin receptor protein that is encoded by a nucleic acid molecule that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency, said fragment being able to bind to a BT-toxin.
A method to produce BT-toxin receptor protein, or a fragment thereof, said method comprising the steps of:
- i) culturing a cell that has been altered to contain a nucleic acid molecule that encodes a BT-toxin receptor protein, of BT-toxin binding fragment thereof, wherein said cell has been altered to contain a nucleic acid molecule selected from the group consisting of a) a nucleic acid molecule that encodes the amino acid sequence of SEQ ID No:2, b) a nucleic acid molecule that encodes a fragment of the amino acid sequence of SEQ ID No:2 that binds to a BT toxin, c) a nucleic acid molecule encoding a BT-toxin receptor that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency, and d) a nucleic acid molecule that encodes a fragment of a BT-toxin receptor that hybridizes to the nucleic acid sequence of SEQ ID No:1 under high stringency and that binds to a BT toxin, under condition in which said nucleic acid molecule is expressed and
- ii) isolating said BT-toxin receptor protein or fragment.
Dependencies are inferred from the claim text.