Nucleic acid fragments for the identification of dechlorinating bacteria
AssigneeE. I. Du Pont de Nemours and Company
InventorsRichard C. Ebersole, Edwin R. Hendrickson
Life of this patent
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Abstract
A unique 16S rRNA profile derived from Dehalococcoides ethenogenes has been identified and isolated. The profile contains several nucleic acid fragments that are linked to dechlorinating activity. These sequences are set forth in SEQ ID NO:1, SEQ ID NO:8, SEQ ID NO:30, and SEQ ID NO:34.
Claims32
An isolated 16S rDNA sequence indicative of a dechlorinating bacterial organism selected from the group consisting of;
- (a) SEQ ID NO:94 and SEQ ID NO:95
- (b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions:
- 0.1× SSC, 0.1% SDS at 65° C.;
- or
- an isolated nucleic acid molecule that is completely complementary to (a) or (b).
An isolated 16S rDNA sequence indicative of a dechlorinating bacterial organism selected from the group consisting of:
- (a) SEQ ID NOs:61-75 and SEQ ID NO:
- 's 77-93;
- (b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions:
- 0.1× SSC, 0.1% SDS at 65° C.;
- or
- an isolated nucleic acid molecule that is completely complementary to (a) or (b).
A diagnostic nucleic acid gene fusion useful in Denaturing Gradient Gel Electrophoresis having the general structure:
- DS-GC, wherein:
- (i) SS is a signature sequence selected from the group consisting of SEQ ID NOs:9-29, SEQ ID NO:30, SEQ ID NO:34, SEQ ID NO:94, SEQ ID NO:
- 95, SEQ ID NOs:35-75, and SEQ ID NOs:
- 77-93;
- and
- (ii) GC is a GC clamp sequence having the sequence as set forth in SEQ ID NO:
- 76.
An isolated 16S rDNA sequence indicative of a dechlorinating bacterial strain selected from the group consisting of:
- (a) SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 SEQ ID NO:8, SEQ ID NO:30, and SEQ ID NO:34;
- (b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions:
- 0.1× SSC, 0.1% SDS at 65° C.;
- and
- (c) an isolated nucleic acid molecule that is completely complementary to (a) or (b).
An isolated 16S rDNA sequence indicative of a dechlorinating bacterial strain selected from the group consisting of:
- (a) SEQ ID NOs:9-29 and SEQ ID NOs:35-60;
- (b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions:
- 0.IX SSC, 0.1% SDS at 65° C.;
- and
- (c) an isolated nucleic acid molecule that is completely complementary to (a) or (b).
Dependencies are inferred from the claim text.