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US 2003/0073136 A1Published application
Method of and apparatus for performing flow cytometric measurements
OwnerGerd Luedke, Bernd Nawracalainventors
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OwnerGerd Luedke, Bernd Nawracalainventors
Estimating the term…
Analyte cells with different properties flow through a flow cytometric detector arrangement. The number of analyte cells having the different properties is determined. To compensate for errors due to two or more analyte cells being simultaneously in the cytometric cell, the actual number of analyte cells with a specific property is calculated from the measured values of cells with this specific property by using a statistical model about the probability of simultaneous occurrences of cells in the cytometric cell. A data processing arrangement has a memory including the model and receives as input data the measured analyte cell numbers and uses an experimentally determined coincidence rate for calculating actual analyte cell numbers.
A method of performing flow cytometric measurements wherein analyte cells with different properties are flowing through a flow cytometric cell of a detector arrangement, and wherein the number of analyte cells having specific different properties is determined, comprising compensating for errors due to two or more analyte cells being simultaneously detected as being in the flow cytometric cell by calculating the actual number of analyte cells with a specific property from measured values of the number of analyte cells with the specific property, the calculation being performed by using a statistical model about the probability of simultaneous occurrences of analyte cells being detected by the detector arrangement.
An apparatus for performing flow cytometric measurements, comprising a flow cytometric cell of a detector arrangement, the flow cytometric cell being arranged to be responsive to flowing analyte cells having different properties, a processor arrangement coupled with the detector arrangement for (a) determining the numbers of analyte cells having each of the properties, and (b) compensating for errors due to two or more analyte cells being detected as simultaneously in the flow cytometric cell, the processor arrangement being arranged for performing the compensation by calculating the actual numbers of analyte cells with a specific property, the data processor arrangement including a statistical model about the probability of simultaneous occurrences of analyte cells being detected as being simultaneously in the flow cytometric cell, the statistical model being part of the arrangement for calculating the actual numbers of analyte cells with the specific property.
A memory for use in a computer adapted to be responsive to flow cytometric measurements, derived from a detector arrangement arranged to be responsive to analyte cells flowing through a flow cytometric cell, wherein the analyte cells have different properties, the memory storing a program for enabling the computer to (a) determine the numbers of analyte cells having each of the properties, (b) compensate for errors due to two or more analyte cells being detected as simultaneously in the flow cytometric cell, and (c) perform the compensation by calculating the actual numbers of analyte cells with a specific property;
Dependencies are inferred from the claim text.