US 2003/0069701 A1 · Probe designing apparatus and probe designing method

Abstract

Where a DNA chip corresponding to a bacterium that is a subsequently added identification object is produced, temporal and pecuniary cost is reduced, and even where a probe unique to the DNA sequence of an identification object cannot be designed, precision in identifying DNA comprised in a sample is maximized. First, on designing a probe, a plurality of different probes are prepared to one kind of bacterium. Where some probes come to be not available by addition of bacteria that are new identification objects, identification is carried out using the remaining probes. Where a probe unique to an identification target bacterium cannot be designed, a probability of correct identification is increased by using multiple probes. Moreover, in consideration with a possibility that multiple kinds of bacteria simultaneously exist, a combination of probes that maximizes a probability of correct identification is selected.

Claims16

Dependent claims

A probe designing apparatus, which designs a probe set for identifying multiple kinds of targets that are identification objects by the medium of hybridization, said probe designing apparatus comprising,

an identification object target registration processing unit for registering the information of an identification target comprising a base sequence;
a probe selection processing unit for selecting, as a probe, a partial sequence unique to each of the registered targets, from partial sequences of the base sequence;
and
a multiple probes substitution processing unit for selecting a combination of multiple probes as a substituted probe to said target, in a case where, in said probe selection processing unit, there remains a target for which a unique partial sequence could not be selected.

1 more element

a means for reading the information of the existing probe set for identifying multiple kinds of targets;
an identification object target addition processing unit for reading information comprising the base sequence of a new target that is to be added to identification objects;
a probe reanalysis processing unit for detecting a target which becomes difficult to be identified by the probes in said existing probe set due to the addition of said new target;
a probe addition processing unit for adding a probe for identifying said new target and a probe for identifying the target detected with said probe reanalysis processing unit;
a multiple probes substitution processing unit for selecting a combination of multiple probes as a substituted probe to said target, in a case where, in said probe addition processing unit, there remains a target for which a unique probe could not be selected;
and
a probe updation processing unit for updating a probe set by reflecting processing results from said probe addition processing unit and/or said multiple probes substitution processing unit.

4 more elements

Dependencies are inferred from the claim text.

Probe designing apparatus and probe designing method

Life of this patent

Probe designing apparatus and probe designing method

Life of this patent

Abstract

Claims16