T1-R ligand III
AssigneeHuman Genome Sciences, Inc.
InventorsJian Ni, Reiner L. Gentz, Steven M. Ruben
Life of this patent
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Abstract
The present invention relates to a novel T1-R ligand III protein which is a member of the IL-1RI ligand-like family. In particular, isolated nucleic acid molecules are provided encoding the human T1-R ligand III protein. T1-R ligand III polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of T1-R ligand III activity. Also provided are diagnostic methods for detecting immune system-related disorders and therapeutic methods for treating immune system-related disorders.
Claims24
An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding the T1-R ligand III polypeptide having the amino acid sequence at positions −24 to 139 of SEQ ID NO:2 or the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97859
- (b) a nucleotide sequence encoding the T1-R ligand III polypeptide having the amino acid sequence at positions −24 to 191 of SEQ ID NO:4 or the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97858
- (c) a nucleotide sequence encoding the T1-R ligand III polypeptide having the amino acid sequence at positions −23 to 139 of SEQ ID NO:2 or the complete amino acid sequence excepting the N-terminal methionine encoded by the cDNA clone contained in ATCC Deposit No. 97859
- (d) a nucleotide sequence encoding the T1-R ligand III polypeptide having the amino acid sequence at positions −23 to 191 of SEQ ID NO:4 or the complete amino acid sequence excepting the N-terminal methionine encoded by the cDNA clone contained in ATCC Deposit No. 97858
- (e) a nucleotide sequence encoding the mature form of the T1-R ligand III polypeptide having the amino acid sequence at positions 1 to 139 in SEQ ID NO:2, or as encoded by the cDNA clone contained in the ATCC Deposit No, 97859;
- (f) a nucleotide sequence encoding the mature form of the T1-R ligand III polypeptide having the amino acid sequence at positions 1 to 191 in SEQ ID NO:4, or as encoded by the cDNA clone contained in the ATCC Deposit No. 97858;
- (g) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d), (e) or (f) above.
An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues n-139 of SEQ ID NO:2, where n is an integer in the range of −23-15;
- (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues −23-m of SEQ ID NO:2, where m is an integer in the range of 129 to 138;
- (c) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues n-m of SEQ D NO:2, where n and m are integers as defined respectively in (a) and (b) above;
- and
- (d) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete T1-R ligand III amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97859 wherein said portion excludes from 1 to about 14 amino acids from the amino terminus of said complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97859;
- (e) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete T1-R ligand III amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97859 wherein said portion excludes from 1 to about 10 amino acids from the carboxy terminus of said complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97859;
- and
- (f) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete T1-R ligand III amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97859 wherein said portion include a combination of any of the amino terminal and carboxy terminal deletions in (d) and (e), above.
An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues n-139 of SEQ ID NO:4, where n is an integer in the range of −23-15;
- (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues −23-m of SEQ ID NO:4, where m is an integer in the range of 181 to 190;
- (c) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues n-m of SEQ ID NO:4, where n and m are integers as defined respectively in (a) and (b) above;
- and
- (d) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete T1-R ligand III amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97858 wherein said portion excludes from 1 to about 14 amino acids from the amino terminus of said complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97858;
- (e) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete T1-R ligand III amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97858 wherein said portion excludes from 1 to about 10 amino acids from the carboxy terminus of said complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97858;
- and
- (f) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete T1-R ligand III amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97858 wherein said portion include a combination of any of the amino terminal and carboxy terminal deletions in (d) and (e), above.
An isolated T1-R ligand III polypeptide comprising an amino acid sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) the amino acid sequence positions −23 to 139 of SEQ ID NO:2 or amino acid sequence positions −23 to 191 of SEQ ID NO:4 or the complete T1-R ligand III amino acid sequence excepting the N-terminal methionine encoded by the cDNA clone contained in ATCC Deposit No. 97859 or in ATCC Deposit No. 97858;
- and
- (b) the amino acid sequence of the mature T1-R ligand III polypeptide having the amino acid sequence at positions 1 to l 39 in SEQ ID NO:2 or amino acid sequence at positions 1 to 191 in SEQ ID NO:4, or as encoded by the cDNA clone contained in the ATCC Deposit No. 97859 or in ATCC Deposit No. 97858.
An isolated polypeptide comprising an epitope-bearing portion of the T1-R ligand III protein, wherein said portion is selected from the group consisting of:
- a polypeptide comprising amino acid residues from about 1 to about 10, about 30 to about 40, about 45 to about 57, about 89 to about 100, about 120 to about 132, and about 150 to about 162 of SEQ ID NO:2.
An isolated polypeptide comprising an epitope-bearing portion of the T1-R ligand III protein, wherein said portion is selected from the group consisting of:
- a polypeptide comprising amino acid residues from about 1-10, about 30 to about 40, about 45 to about 57, about 89 to about 100, about 120 to about 132, about 155 to about 165, and about 201 to about 212.
An isolated nucleic acid molecule comprising a polynucleotide having a sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) the nucleotide sequence of clone HETDW91R (SEQ ID NO:6);
- (b) the nucleotide sequence of clone HSRDN17R (SEQ ID NO:7);
- (c) the nucleotide sequence of a portion of the sequence shown in FIGS. 1A-1B (SEQ ID NO:1) wherein said portion comprises at least 50 contiguous nucleotides from a nucleotide sequence selected from the group consisting of about 1 to about 100, about 450 to about 615, and about 690 to about 1836;
- and
- (d) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b) or (c) above.
An isolated nucleic acid molecule comprising a polynucleotide having a sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) the nucleotide sequence of clone HETDW91R (SEQ ID NO:6);
- (b) the nucleotide sequence of clone HSRDN17R (SEQ ID NO:7);
- (c) the nucleotide sequence of clone HASAA31R (SEQ ID NO:8);
- (d) the nucleotide sequence of clone HPFCQ85R (SEQ ID NO:9);
- and
- (e) the nucleotide sequence of a portion of the sequence shown in FIGS. 2A-2B (SEQ ID NO:3) wherein said portion comprises at least 50 contiguous nucleotides from nucleotide about 1 to about 100, about 1000 to about 1390, and about 1430 to about 1513;
- and
- (f) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d) or (e) above.
Dependencies are inferred from the claim text.