Determination of an analyte using two labels
AssigneeBoehringer Mannheim GmbH
InventorsUrsula Giesen, Peter Wenzig, Gunter Ziegler
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Abstract
The combination of two mechanisms of detection in a single device yields a method which allows the simple quantification of analyte determinations. Suitable formats and reagent kits are suggested.
Claims18
Method for the determination of an analyte in a sample by
- incubation of the sample with at least two probes, of which at least one is specific for the analyte to be determined and whereby the at least two probes carry different labeling groups,
- independent generation of an electromagnetic signal by each different labeling group and
- evaluation of a signal generated as an indication of the presence of an amount of the analyte.
Method according to claim one, characterized in that the second and possibly further probes are specific to a second or possibly other components contained in the sample.
Reagent kit for the determination of an analyte containing in one or separate containers two or more probes of which at least one is specific for the detection of the analyte whereby the at least two probes carry different luminescent labeling groups.
Use of two probes, which can generate different luminescent signals for the quantitative detection of an analyte in a sample.
A method for the detection of an analyte-nucleic acid in a sample comprising
- a) Amplification of at least a partial sequence of the analyte nucleic acid as well as a partial sequence of a defined amount of a standard nucleic acid in the sample,
- b) Addition of an analyte-specific probe and a standard nucleic acid specific probe, which carry different labeling groups to those in the sample containing the amplified nucleic acids,
- c) Removal of the mixture from b) from the measuring cell ensuring that the nucleic acids and probes remain in the measuring cell,
- d) Transfer of the nucleic acids in the mixture in b) to a measuring cell,
- e) Excitation of the first labeling group such that a signal is produced,
- f) Measurement of the signal generated in d) by excitement,
- g) Excitation of the second labeling group to generate a signal,
- h) Measurement of the signal generated by excitation in g)
- i) Comparison of the measured signal for the detection of the analyte nucleic acid.
Method for the determination of at least two analytes in a sample by
- Incubation of the sample with at least two probes, of which at least one is specific for the analyte to be determined and whereby such probes carry different labeling groups,
- independent generation of an electromagnetic signal for the detection of each labeling group and
- Evaluation of the signal generated as an indication of the presence or the amount of the analyte.
Dependencies are inferred from the claim text.