Nucleotide sequences coding for variable regions of beta chains of human T lymphocyte receptors, corresponding peptide segments and the diagnostic and therapeutic uses
AssigneeAventis Pharma S.A.
InventorsThierry Hercend, Frederic Triebel, Sergio Roman-Roman, Laurent Ferradini
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Abstract
The present invention relates to new nucleotide sequences coding for variable regions of β chains of human T lymphocyte receptors, corresponding peptide segments and the diagnostic and therapeutic uses.
Claims31
Nucleotide sequences coding for the variable regions of β chains of human T lymphocyte receptors, corresponding to cDNAs containing nucleotide sequences chosen from any one of the Vβ segments corresponding to one of the sequences SEQ ID No. 2 to 19, and the sequences which differ from them by one or more nucleotides.
Nucleotide sequences coding for the variable regions of β chains of human T lymphocyte receptors, corresponding to the cDNAs corresponding to all or part of the nucleotide sequences chosen from any one of the Vβ segments corresponding to one of the sequences SEQ ID Nos. 2 to 5, and the sequences which differ from them by one or two nucleotides.
Nucleotide sequences coding for the variable regions of β chains of human T lymphocyte receptors, corresponding to the cDNAs corresponding to one of the nucleotide sequences chosen from any one of the Vβ segments corresponding to one of the sequences SEQ ID Nos. 6 to 15, the sequences which differ from them by one or two nucleotides and fragments of the latter.
Nucleotide sequences coding for the variable regions of β chains of human T lymphocyte receptors, corresponding to the cDNAs corresponding to all or part of the nucleotide sequences chosen from any one of the Vβ segments corresponding to one of the sequences
- 1 to 195 of SEQ ID No. 16
- 1 to 99 of SEQ ID No. 17
- 1 to 113 of SEQ ID No. 18
- 1 to 186 of SEQ ID No. 19
- and the sequences which differ from them by one or two nucleotides.
Oligonucleotides, which can be used as primers for the amplification of DNA corresponding to variable regions of β chains of T-cell receptors, chosen form the sequences SEQ ID No. 25 to 48.
Kit according to claim 28 or claim 29, containing:
- a) the group of 24 oligonucleotides according to claim
- b) a Cβ primer chosen form the sequences corresponding sequences SEQ ID 49 and 50,
- c) a pair of control primers for the β-actin having a sequence corresponding to sequences SEQ ID No. 52 and 53 respectively,
- d) a Cβ probe corresponding to the sequence SEQ ID No. 1
- e) a control probe for the β-actin corresponding to the sequence SEQ ID No. 54.
Hybridomas RO-73 and JU-74 producing anti Vβ 13 monoclonal antibodies deposited at the CNCM on the 12th February 1992 under the numbers I-1172 and I-1173.
Dependencies are inferred from the claim text.