Method and apparatus for scoring the uptake of markers in cells
AssigneeChromaVision Medical Systems, Inc.
InventorJose De La Torre-Bueno
Life of this patent
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Abstract
In an embodiment, an imager is used to generate an image of a sample, e.g., a tissue sample including diaminobenzidine tetrahydrochloride (DAB) stained cells. A user selects a region to be scored in the image. A mean intensity value of a selected color is determined from the pixels in the selected region. The selected color may be the complement of a color to be scored, which, in the case of a DAB test, is blue. A score is generated in response to the mean intensity value using a stored calibration curve.
Claims30
A method comprising:
- generating an image of a sample;
- selecting a region in the sample for scoring;
- determining a mean intensity value of a selected color in the region;
- and
- generating a score in response to the mean intensity value using a calibration curve.
An apparatus comprising:
- an imager operative to generate an image comprising a plurality of pixels;
- an intensity analyzer operative to determine a mean intensity value of a selected color in a plurality of pixels in a selected region of the image;
- a memory device operative to store a calibration curve;
- and
- a correlator operative to generate a score in response to a determined mean intensity value for the selected region using the calibration curve.
An article comprising a machine-readable medium which stores machine-executable instructions, the instructions operative to cause a machine to:
- generate an image of a sample;
- select a region in the sample for scoring;
- determine a mean intensity value of a selected color in the region;
- and
- generate a score in response to the mean intensity value using a calibration curve.
Dependencies are inferred from the claim text.