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US 2003/0003534 A1Published application
Methods and compositions for extracting proteins from cells
AssigneeAkzo Nobel N.V.
InventorScot R. Sheppard
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Abstract
The present invention relates to a process of releasing a protein, recombinant or otherwise, from a cell. The process of the present invention involves contacting a host cell containing a protein of interest with a solution comprising one or more detergents and one or more reducing agents. The methods of the invention are particularly suitable to large scale production of recombinant products.
Claims13
A method of releasing a protein of interest from host cells comprising contacting the cells with a reducing agent and a detergent, wherein the detergent is an amphipathic charged amine or an amphipathic charged amine oxide.
Dependencies are inferred from the claim text.