Plant sugar transport proteins
AssigneeE. I. Du Pont de Nemours and Company
InventorsStephen M. Allen, William D. Hitz, Anthony J. Kinney, Scott V. Tingey
Life of this patent
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a sugar transport protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sugar transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sugar transport protein in a transformed host cell.
Claims15
An isolated nucleic acid fragment encoding all or a substantial portion of an Arabidopsis thaliana-like sugar transport protein comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:2, 4, 6, 8, 10, 12, 14 and 16;
- (b) an isolated nucleic acid fragment that is substantially similar to an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:2, 4, 6, 8, 10, 12, 14 and 16;
- and
- (c) an isolated nucleic acid fragment that is complementary to (a) or (b).
An Arabidopsis thaliana-like sugar transport protein polypeptide comprising all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:2, 4, 6, 8, 10, 12, 14 and 16.
An isolated nucleic acid fragment encoding all or a substantial portion of an Beta vulgaris-like sugar transport protein comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:18, 20, 22, 24, 26 and 28;
- (b) an isolated nucleic acid fragment that is substantially similar to an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:18, 20, 22, 24, 26 and 28;
- and
- (c) an isolated nucleic acid fragment that is complementary to (a) or (b).
An Beta vulgaris sugar transport protein polypeptide comprising all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:18, 20, 22, 24, 26 and 28.
A method of obtaining a nucleic acid fragment encoding a substantial portion of an amino acid sequence encoding a sugar transport protein comprising:
- (a) synthesizing an oligonucleotide primer corresponding to a portion of the sequence set forth in any of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25 and 27;
- and
- (b) amplifying a cDNA insert present in a cloning vector using the oligonucleotide primer of step (a) and a primer representing sequences of the cloning vector
- wherein the amplified nucleic acid fragment encodes a substantial portion of an amino acid sequence encoding a sugar transport protein.
Dependencies are inferred from the claim text.