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US 2002/0178467 A1Published application
Plastid transit peptide sequences for efficient plastid targeting
OwnerKatayoon Deheshinventor
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Abstract
Novel nucleic acid sequences encoding plastid transit peptides are provided. The plastid transit peptide is capable of translocating a protein operably linked to the transit peptide, to a plant cell plastid. Also considered are arnino acid and nucleic acid sequences of the plastid transit peptides of the invention and the use of such sequences to produce DNA constructs. Provided are methods for the increased translocation of proteins to a plant cell plastid using the nucleic acid sequences of the present invention.
Claims13
A recombinant DNA construct comprising:
- a promoter molecule that functions in plant cells, operably linked to a heterologous DNA molecule encoding a plastid transit peptide of a Cuphea acyl-ACP thioesterase, operably linked to a heterologous DNA molecule encoding a protein, operably linked to a DNA molecule providing 3′ termination functions.
A recombinant DNA construct comprising:
- a promoter molecule that functions in plant cells, operably linked to a heterologous DNA molecule encoding a plastid transit peptide with at least 90% identity to SEQ ID NO:2, operably linked to a heterologous DNA molecule encoding a protein, operably linked to a DNA molecule providing 3′ termination functions.
A recombinant DNA construct comprising:
- a promoter molecule that functions in plant cells, operably linked to a heterologous DNA molecule encoding a plastid transit peptide of a Cuphea palustris FatB2 acyl-ACP thioesterase, operably linked to a heterologous DNA molecule encoding an acyl-ACP thioesterase, operably linked to a DNA molecule providing 3′ termination functions.
Dependencies are inferred from the claim text.