Proton transporters and uses in plants
AssigneeWhitehead Institute for Biomedical Research
InventorsRoberto A. Gaxiola, Gerald R. Fink, Seth L. Alper
Life of this patent
Estimating the term…
Loading patent…
AssigneeWhitehead Institute for Biomedical Research
InventorsRoberto A. Gaxiola, Gerald R. Fink, Seth L. Alper
Estimating the term…
The present invention relates to a transgenic plant which is tolerant to a salt, comprising one or more plant cells transformed with exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant. Also encompassed by the present invention are transgenic progeny and seeds of the transgenic plants described herein. Progeny transgenic plant grown from seed are also described. The present invention also relates to a construct comprising an AVP1 gene operably linked to a chimeric promoter designed to overexpress AVP1 or designed to down regulate endogenous pyrophosphatase. Plant cells (e.g., root cells, stem cell, leaf cells) comprising exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant cell are also the subject of the present invention. Also encompassed by the present invention are methods of making a transgenic plant described herein. Transgenic plants produced by the methods of making a transgenic plant as described herein are also a subject of the present invention. The present invention also relates to a method of bioremediating soil, a method of increasing the yield of a plant, a method of making a plant which is larger than its corresponding wild type plant, and a method of producing a transgenic plant which grows in salt water comprising introducing into one or more cells of a plant nucleic acid which alters expression of vacuolar pyrophosphatase in the plant. The transgenic plants of the present invention can also be used to produce double transgenic plants which are tolerant to a salt.
A transgenic plant which is tolerant to a salt, comprising one or more plant cells transformed with exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant.
A transgenic plant which grows in a concentration of a salt that inhibits growth of a corresponding non-transgenic plant.
A transgenic plant which is tolerant to a salt comprising an exogenous nucleic acid construct which is designed to overexpresses AVP1 or designed to down regulate endogenous pyrophosphatase.
A construct comprising an AVP1 gene operably linked to a chimeric promoter designed to overexpress AVP1 or designed to down regulate endogenous pyrophosphatase.
A transgenic plant obtained by introducing into a plant exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant.
Plant cells comprising exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant cell.
A method of making a transgenic plant which is tolerant to a salt comprising introducing into one or more cells of a plant exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant to yield transformed cells in the plant, thereby producing a transgenic plant which is tolerant to the salt.
A method of making a transgenic plant which is tolerant to a salt comprising introducing into one or more cells of a plant a nucleic acid construct which is designed to overexpress AVP1 to yield transformed cells thereby producing a transgenic plant which is tolerant to the salt.
A method of making a transgenic plant which is larger than its corresponding wild type plant comprising introducing into one or more cells of a plant nucleic acid which alters expression of vacuolar pyrophosphatase in the plant to yield transformed cells, thereby producing a transgenic plant which is larger than its corresponding wild type plant.
A method of bioremediating soil comprising growing one or more transgenic plants and/or progeny thereof in the soil, wherein the transgenic plants and/or progeny thereof comprise exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant.
A method of increasing the yield of a plant comprising introducing into one or more cells of a plant nucleic acid which alters expression of vacuolar pyrophosphatase in the plant to yield transformed cells, thereby increasing the yield of the plant.
A method of removing cations from a medium which can support plant growth comprising growing one or more transgenic plants and/or progeny thereof in the medium, wherein the transgenic plants and/or progeny thereof comprise exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant.
A method of producing a transgenic plant which grows in salt water comprising introducing into one or more cells of a plant exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant to yield transformed cells, thereby producing a transgenic plant which can grow in salt water.
A transgenic plant which is tolerant to a salt, comprising one or more plant cells transformed with exogenous nucleic acid which alters expression of a vacuolar pyrophosphatase and an Na+/H+ antiporter in the plant.
A method of making a transgenic plant having increased flower size compared to its corresponding wild type plant comprising introducing into one or more cells of a plant nucleic acid which alters expression of vacuolar pyrophosphatase in the plant to yield transformed cells, thereby producing a transgenic plant having increased flower size compared to its corresponding wild type plant.
Dependencies are inferred from the claim text.