Transgenic fish with tissue-specific expression
AssigneeMedical College of Georgia Research Institute, Inc.
InventorShuo Lin
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Abstract
Disclosed are transgenic fish, and a method of making transgenic fish, which express transgenes in stable and predictable tissue- or developmentally-specific patterns. The transgenic fish contain transgene constructs with homologous expression sequences. Also disclosed are methods of using such transgenic fish. Such expression of transgenes allow the study of developmental processes, the relationship of cell lineages, the assessment of the effect of specific genes and compounds on the development or maintenance of specific tissues or cell lineages, and the maintenance of lines of fish bearing mutant genes.
Claims40
A transgenic fish the cells of which contain an exogenous construct, wherein the construct comprises homologous expression sequences operably linked to a sequence encoding an expression product, wherein the expression product is expressed only in specific cell lineages.
A method of making transgenic fish, the method comprising
- (a) introducing an exogenous construct into an embryonic cell of a first fish, wherein the construct comprises homologous expression sequences operably linked to a sequence encoding an expression product, and
- (b) allowing the egg cell or embryonic cells to develop into a second fish, wherein the expression product is expressed only in specific cell lineages of the second fish.
A method of identifying regulatory elements in sequences upstream of a gene of interest, the method comprising
- (a) introducing members of a set of exogenous constructs into separate embryonic cells, wherein each member of the set of constructs comprises a sequence encoding an expression product operably linked to upstream sequences of a homologous gene of interest, wherein the different members of the set have different regions of the upstream sequences deleted,
- (b) allowing the embryonic cells to develop into fish,
- (c) detecting the expression product in the fish or progeny of the fish,
- (d) determining which regions of the upstream sequences are needed for expression of the expression product.
A nucleic acid construct comprising expression sequences derived from fish operably linked to a sequence encoding an expression product, wherein the expression sequences comprise a promoter operably linked to a enhancer, wherein the expression product is expressed only in specific cell lineages.
Dependencies are inferred from the claim text.