HER-2/neu fusion proteins
AssigneesCorixa Corporation, SmithKline Beecham Biologicals s.a.
InventorsMartin A. Cheever, Dirk Gheysen
Life of this patent
Estimating the term…
Abstract
The present invention is generally directed to HER-2/neu fusion proteins, nucleic acid molecules encoding HER-2/neu fusion proteins, Viral vectors expressing HER-2/neu fusion proteins, and pharmaceutical compositions (e.g., vaccines) comprising the HER-2/neu fusion proteins and/or nucleic acid molecules encoding the HER-2/neu fusion proteins. The present invention is also directed to methods of treating or preventing cancer by eliciting or enhancing an immune response to the HER-2/neu protein, including for uses in the treatment of malignancies associated with the HER-2/neu oncogene.
Claims92
An isolated protein comprising a HER-2/neu extracellular domain fused to a HER-2/neu phosphorylation domain, wherein the protein is capable of producing an immune response in a warm-blooded animal.
An isolated protein comprising a HER-2/neu extracellular domain fused to a fragment of the HER-2/neu phosphorylation domain, wherein the protein is capable of producing an immune response in a warm-blooded animal.
An isolated protein comprising a HER-2/neu extracellular domain fused to a HER-2/neu intracellular domain, wherein the protein is capable of producing an immune response in a warm-blooded animal.
A method for removing tumor cells from a biological sample, the method comprising the step of contacting a biological sample with T cells that specifically react with a HER-2/neu fusion protein, wherein the fusion protein comprises an amino acid sequence that is encoded by a polynucleotide sequence selected from the group consisting of:
- (i) polynucleotides recited in any one of SEQ ID NO:
- 8, 33, or 58;
- and
- (ii) complements of the foregoing polynucleotides;
- wherein the step of contacting is performed under conditions and for a time sufficient to permit the removal of cells expressing the antigen from the sample.
Dependencies are inferred from the claim text.